Human glucocorticoid receptor-β,GR-β ELISA Kit

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產品詳情

產品描述：

人糖皮質激素受體β(GR-β)酶聯免疫試劑盒（CSB-E08889h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的GR-β含量。GR-β即糖皮質激素受體β，是核受體超家族成員。它能拮抗GR-α轉錄活性，影響糖皮質激素效應。研究發現其在哮喘等炎癥性疾病中異常表達，深入探究其作用機制，可為相關疾病的治療提供新的靶點與思路。試劑盒檢測范圍為39 pg/mL-2500 pg/mL，適用于科研領域對GR-β表達水平的精準分析；可為體外研究GR-β在疾病模型中的表達調控、藥物干預效果評估以及信號通路機制探索提供可靠工具；實驗樣本兼容性廣泛，支持從基礎細胞實驗到動物組織研究的多樣化需求，操作流程標準化，適用于實驗室常規蛋白定量分析本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

lucocorticoid receptor-β,GR-β|lucocorticoid receptor-beta,GR-beta
縮寫：

GR-β
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

39 pg/mL-2500 pg/mL
靈敏度：

9.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	91
	Range %	88-95
1:8	Average %	89
	Range %	85-96

回收率：

The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.