Human dynorphin,Dyn ELISA Kit

Human dynorphin,Dyn ELISA Kit

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中文名稱：

人強(qiáng)啡肽(Dyn)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09128h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人強(qiáng)啡肽(Dyn)酶聯(lián)免疫試劑盒（CSB-E09128h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Dyn含量。人強(qiáng)啡肽（Dyn）是一種具有阿片樣活性的內(nèi)源性肽，主要作用于κ-阿片受體，具有鎮(zhèn)痛、減輕腦水腫、改善缺血后腦記憶障礙等腦保護(hù)作用。其作用機(jī)制主要通過(guò)阿片樣受體途徑和非阿片樣受體途徑實(shí)現(xiàn)，如κ受體介導(dǎo)的鎮(zhèn)痛和NMDA受體途徑的腦保護(hù)作用。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，本試劑盒檢測(cè)范圍為3.12 pg/mL至200 pg/mL，可精準(zhǔn)識(shí)別低豐度靶標(biāo)，適用于神經(jīng)生物學(xué)、疼痛機(jī)制研究或精神疾病動(dòng)物模型中的動(dòng)態(tài)監(jiān)測(cè)。操作簡(jiǎn)便，僅需100 μL樣本即可完成檢測(cè)，配套提供標(biāo)準(zhǔn)品、稀釋液及終止液等完整組分，適用于實(shí)驗(yàn)室規(guī)模的高通量篩查或靶向性研究，為探索Dyn在神經(jīng)信號(hào)傳導(dǎo)、藥物干預(yù)效果評(píng)估等科研領(lǐng)域提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫：

Dyn
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Neuroscience
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Dyn in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	90-96
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of human Dyn spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-99
EDTA plasma (n=4)	100	97-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.537	2.576	2.557	2.451
100	2.141	2.164	2.153	2.047
50	1.778	1.791	1.785	1.679
25	1.405	1.455	1.430	1.324
12.5	1.012	1.053	1.033	0.927
6.25	0.604	0.624	0.614	0.508
3.12	0.348	0.357	0.353	0.247
0	0.105	0.107	0.106

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

In major depression, increased kappa and mu opioid receptor levels are associated with immune activation Al-Hakeim H K, et al,Acta Neuropsychiatrica,2019
Evaluation of dynorphin and kappa-opioid receptor level in the human blood lymphocytes and plasma: Possible role as a biomarker in severe opioid use disorder Vousooghi N, et al,Drug and Alcohol Dependence,2019
In Major Depression, Increased Serum Dynorphin and Kappa Opioid Receptor Levels are Positively Associated with Mu Opioid Receptor Levels and Immune Activation and Are Attenuated by Nicotine Dependence Hussein Al-Hakeim, et al,Preprints,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.