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中文名稱:人多巴胺(DA)酶聯免疫試劑盒
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貨號:CSB-E06888h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人多巴胺(DA)酶聯免疫試劑盒(CSB-E06888h)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的DA含量。DA即多巴胺,是一種重要神經遞質。其在調節運動、情感、認知等生理過程中發揮關鍵作用,異常時會引發如帕金森病、精神分裂癥等多種疾病。相關研究聚焦于其合成、釋放、代謝機制及與受體相互作用,為開發針對性治療藥物提供方向。試劑盒檢測范圍為0.5 ng/mL-100 ng/mL,該產品支持多樣本類型分析,便于研究人員探索不同生物基質中多巴胺的動態變化,如神經藥理學研究中藥物干預對神經遞質釋放的影響、疾病模型動物腦組織多巴胺代謝評估,或體外培養神經元/神經母細胞瘤細胞的多巴胺分泌水平監測。適用于神經生物學、行為學及分子機制研究中的多巴胺定量分析需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:DA
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates
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檢測范圍:0.5 ng/mL-100 ng/mL
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靈敏度:0.25 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Neuroscience
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 90-98 1:2 Average % 90 Range % 86-94 1:4 Average % 90 Range % 86-94 1:8 Average % 102 Range % 97-105 -
回收率:
The recovery of human DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-98 EDTA plasma (n=4) 94 89-98 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 100 0.220 0.216 0.218 25 0.328 0.337 0.333 8 0.512 0.532 0.522 2 0.884 0.864 0.874 0.5 1.230 1.217 1.224 0 1.903 2.021 1.962 -
數據處理:
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貨期:3-5 working days
引用文獻
- A??larda Kullan?lan Koruyucu ve Adjuvan Maddelerin Olas? Toksik Etkilerinin De?erlendirilmesi ME ?ZTüRK,/,2022
- Impact of saffron (Crocus Sativus Linn) supplementation and resistance training on markers implicated in depression and happiness levels in untrained young males BH Moghadam,Physiology & Behavior,2021
- Hydrogen sulphide ameliorates dopamine‐induced astrocytic inflammation and neurodegeneration in minimal hepatic encephalopathy W Zhuge,Journal of Cellular and Molecular Medicine,2020
- Wearable Corneal Biosensors Fabricated from PEDOT Functionalized Sulfur‐Doped Graphene for Use in the Early Detection of Myopia W Zhang,Advanced science news,2020
- Overeating and food addiction in Major Depressive Disorder: Links to peripheral dopamine Jessica G,Appetite,2020
- Exercise enhances the effectiveness of vitamin D therapy in rats with Alzheimer’s disease: emphasis on oxidative stress and inflammation Medhat E, et al,Metabolic Brain Disease,2019
- Creation of cellular models and functional investigation of PARK2 Copy Number Variants (CNVs) associated with Attention-Deficit/Hyperactivity Disorder (ADHD) Palladino VS,/,2019
- Proof of concept studies exploring the safety and functional activity of human parthenogenetic-derived neural stem cells for the treatment of Parkinson's disease. Gonzalez R.et al,Cell Transplant.,2015
- Deriving dopaminergic neurons for clinical use. A practical approach Gonzalez R et al,Sci Rep.,2013
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