Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

Human cleaved microtubule-associated protein tau (C-MAPT/C-TAU) ELISA kit

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中文名稱：

人裂解微管相關(guān)蛋白tau(C-MAPT/C-TAU)酶聯(lián)免疫試劑盒
貨號：

CSB-ECL013481H
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人裂解微管相關(guān)蛋白tau(C-MAPT/C-TAU)酶聯(lián)免疫試劑盒（CSB-ECL013481H）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、腦脊液（CSF）、組織勻漿、細(xì)胞裂解物樣本中的C-MAPT/C-TAU含量。C-MAPT/C-TAU是研究tau蛋白的基因，tau蛋白是一種參與微管穩(wěn)定的微管相關(guān)蛋白。tau蛋白的異常磷酸化和聚集與阿爾茨海默病等神經(jīng)退行性疾病密切相關(guān)。研究C-MAPT/C-TAU有助于理解tau蛋白在神經(jīng)退行性疾病中的作用機制，為開發(fā)新的治療策略提供理論基礎(chǔ)。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于科研領(lǐng)域?qū)au蛋白的濃度分析；可用于評估疾病模型中的tau蛋白動態(tài)變化、探究神經(jīng)退行機制及藥物干預(yù)效果研究，為神經(jīng)病理學(xué)、分子生物學(xué)實驗提供可靠工具，適用于實驗室基礎(chǔ)研究與轉(zhuǎn)化醫(yī)學(xué)探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

cleaved form of DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
縮寫：

C-MAPT/C-TAU
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, cerebrospinal fluid (CSF), tissue homogenates, cell lysates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C-MAPT/C-TAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	102
	Range %	96-106
1:10	Average %	100
	Range %	90-104
1:20	Average %	95
	Range %	85-99
1:40	Average %	94
	Range %	88-99

回收率：

The recovery of human C-MAPT/C-TAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	100	91-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	3.001	2.889	2.945	2.832
2000	2.114	1.976	2.045	1.932
1000	1.231	1.200	1.216	1.103
500	0.596	0.565	0.581	0.468
250	0.353	0.341	0.347	0.234
125	0.239	0.232	0.236	0.123
62.5	0.171	0.168	0.170	0.057
0	0.111	0.115	0.113

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

A prospective pilot study on serum cleaved tau protein as a neurological marker in severe traumatic brain injury Pandey S.et al,Br J Neurosurg,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.