Human casein alpha s1 (CSN1S1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人酪蛋白α-S1(CSN1S1)酶聯免疫試劑盒（CSB-EL006060HU）為競爭法ELISA試劑盒，定量檢測breast牛奶樣本中的CSN1S1含量。CSN1S1是重要的靶點。它是乳蛋白基因，在乳腺合成酪蛋白過程中起關鍵作用。其研究機制主要圍繞對乳品質影響展開，探究其基因表達調控影響酪蛋白合成量和結構，進而為改善乳品品質、培育優良乳用品種提供理論支撐。試劑盒檢測范圍為37.5 μg/mL-600 μg/mL，適用于乳制品基礎研究、母嬰營養學分析、嬰幼兒配方食品開發中的蛋白質含量比對，以及乳源功能性成分的提取工藝評估等場景，為母乳成分數據庫建立、哺乳期營養干預策略制定提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Alpha casein ELISA Kit; alpha-S1-casein ELISA Kit; CASA ELISA Kit; CASA1_HUMAN ELISA Kit; Casein alpha s1 ELISA Kit; casein; alpha ELISA Kit; casein; alpha-S1; included ELISA Kit; Casoxin-D ELISA Kit; CSN1 ELISA Kit; CSN1S1 ELISA Kit; Csna ELISA Kit; MGC149368 ELISA Kit
縮寫：

CSN1S1
Uniprot No.：

P47710
種屬：

Homo sapiens (Human)
樣本類型：

breast milk
檢測范圍：

37.5 μg/mL-600 μg/mL
靈敏度：

18.75 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CSN1S1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Breast milk(n=4)
1:10	Average %	90
	Range %	86-97
1:20	Average %	93
	Range %	89-99
1:40	Average %	95
	Range %	90-107
1:80	Average %	92
	Range %	87-103

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Important role in the capacity of milk to transport calcium phosphate.; Casoxin D acts as opioid antagonist and has vasorelaxing activity mediated by bradykinin B1 receptors.
基因功能參考文獻：
1. Serine-71 in alpha-S1 casein is phosphorylated in the breast milk. PMID: 28786558
2. Results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food protein alpha casein. PMID: 21361910
3. Casein alpha s1 is expressed in human monocytes and stimulates expression of proinflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) via an immunomodulatory role beyond the nutritional function of CSN1S1 in milk. PMID: 21135174
4. alphas1-Casein may be a potential biomarker for early identification of BPH patients. PMID: 16683014
亞細胞定位：

Secreted.
蛋白家族：

Alpha-casein family
組織特異性：

Mammary gland specific. Secreted in milk.
數據庫鏈接：

HGNC: 2445

OMIM: 115450

KEGG: hsa:1446

STRING: 9606.ENSP00000246891

UniGene: Hs.3155