Human anti-growth hormone(GH) antibody ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人抗生長激素(GH)抗體酶聯(lián)免疫試劑盒（CSB-E09563h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GH Ab含量。人抗生長激素抗體是一種針對生長激素的特異性抗體，其研究背景涉及生長激素在人體中的作用及其相關(guān)疾病的治療。該抗體通過阻斷生長激素的作用，可用于治療因生長激素過量引起的疾病，如肢端肥大癥等。研究機(jī)制主要關(guān)注其與生長激素受體的相互作用及其在體內(nèi)的藥理效應(yīng)。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中對GH相關(guān)疾病的機(jī)制探索、藥物干預(yù)效果評估或內(nèi)分泌調(diào)控研究，例如在動物模型或體外實(shí)驗(yàn)中分析抗體與GH的相互作用，或評估特定病理狀態(tài)下GH抗體表達(dá)動態(tài)變化，為科研人員提供可靠的實(shí)驗(yàn)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GH Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

2 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Developmental Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human anti-GH antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	82-105
1:2	Average %	94
	Range %	84-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	95
	Range %	82-105

回收率：

The recovery of human anti-GH antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	82-105
EDTA plasma (n=4)	93	84-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.