Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Human angiostatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
98
Range %
94-102
1:40
Average %
92
Range %
86-96
1:80
Average %
94
Range %
89-98
1:160
Average %
96
Range %
92-100
回收率:
The recovery of Human angiostatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
88-98
EDTA plasma (n=4)
88
82-94
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.