Human Urocortin-2(UCN2) ELISA kit
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中文名稱：人尿皮素-2(UCN2)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL025552HU
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人尿皮素-2(UCN2)酶聯(lián)免疫試劑盒（CSB-EL025552HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的UCN2含量。UCN2 是一個(gè)靶點(diǎn)。UCN2 即尿皮質(zhì)素 2，屬促腎上腺皮質(zhì)激素釋放因子家族，參與調(diào)節(jié)應(yīng)激反應(yīng)、心血管功能及能量代謝。研究機(jī)制上，其通過(guò)與特定受體結(jié)合激活下游信號(hào)通路，為相關(guān)疾病如心血管病、代謝病的治療帶來(lái)新方向。試劑盒檢測(cè)范圍為6.25 pg/mL-400 pg/mL，該產(chǎn)品適用于體外研究場(chǎng)景，如探究UCN2在代謝調(diào)控相關(guān)細(xì)胞模型中的分泌動(dòng)態(tài)、動(dòng)物實(shí)驗(yàn)中的組織分布分析，或臨床前研究中的生物標(biāo)志物探索，為心血管疾病、肥胖癥及應(yīng)激相關(guān)分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：SRP ELISA Kit; Stresscopin related peptide ELISA Kit; Stresscopin-related peptide ELISA Kit; Ucn II ELISA Kit; Ucn2 ELISA Kit; UCN2_HUMAN ELISA Kit; UCNI ELISA Kit; UCNII ELISA Kit; UR ELISA Kit; Urocortin II ELISA Kit; Urocortin related peptide ELISA Kit; Urocortin-2 ELISA Kit; Urocortin-related peptide ELISA Kit; URP ELISA Kit
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縮寫(xiě)：UCN2
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類(lèi)型：serum, plasma, tissue homogenates, cell culture supernates
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檢測(cè)范圍：6.25 pg/mL-400 pg/mL
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靈敏度：1.56 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human UCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-88 1:2 Average % 90 Range % 85-95 1:4 Average % 97 Range % 92-110 1:8 Average % 93 Range % 86-98 -
回收率：
The recovery of human UCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 104 100-110 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.258 2.302 2.280 2.142 200 1.735 1.696 1.716 1.578 100 1.126 1.101 1.114 0.976 50 0.678 0.637 0.658 0.520 25 0.412 0.434 0.423 0.285 12.5 0.311 0.309 0.310 0.172 6.25 0.243 0.254 0.249 0.111 0 0.137 0.139 0.138 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- The relationship of urocortin-2 with insulin resistance patients having PCOS Temur M.et al,Gynecol Endocrinol.,2016
- High-Sensitivity Sandwich ELISA for Plasma NT-proUcn2: Plasma Concentrations and Relationship to Mortality in Heart Failure Liew OW.et al,Clin Chem.,2016
- Relationship of urocortin-2 with systolic and diastolic functions and coronary artery disease: an observational study /,Anadolu Kardiyol Derg.,2012
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靶點(diǎn)詳情
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功能：Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
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基因功能參考文獻(xiàn)：
- laryngeal cancer cells were enriched in the recurrent chimera COL7A1-UCN2, which potentially affected cancer stem cell transition PMID: 29499655
- examination of the local and systemic cardiovascular effects of urocortin 2 and urocortin 3 in healthy subjects and patients with heart failure PMID: 27275843
- Data show that corticotropin releasing hormone receptor 2 (Crhr2) protein is abundantly expressed in the heart, and plasma Ucn2 levels were higher in patients with heart failure compared to healthy controls, indicating that endogenous Crhr2 is associated with heart failure. PMID: 28550160
- up-regulated in placenta, fetal membranes, and myometrium in labor PMID: 25426872
- Corticotrophin-Releasing Factor (CRF) and the urocortins are potent regulators of the inflammatory phenotype of human and mouse white adipocytes. PMID: 24835211
- Stresscopin-related peptide induces a weaker enhancement of cardiovascular function through corticotropin-releasing factor receptorn 2 than that induced by stresscopin. PMID: 23850799
- Solubilized SUMO-eXact-preproUCN2 was used successfully to generate two high affinity mouse monoclonal antibodies specific to the pro-region of urocortin 2. PMID: 24291344
- UCN2 is significantly associated with AAA and inhibits VSMC proliferation by inducing a G1 cell cycle arrest suggesting a plausible regulatory role in AAA pathogenesis PMID: 24107226
- UCNII caused cell death via different membrane-disrupting mechanisms that involve aggregation & membrane depolarization in bacteria and pore formation in Leishmania even inside macrophages. Innate immune cells produce UCNII in response to infections. PMID: 24249730
- Description of ucn2 posttranslational processing and peptide sequence. Hypoxia and glycosylation, paradigms that might influence secretion or processing of gene products, did not significantly impact hUcn 2 prohormone cleavage. PMID: 23493376
- study showed that Ucn2 and Ucn3 differentially regulate the LPS-induced TNF-alpha and IL-10 expression and secretion in trophoblast explants acting through CRH-R2. Ucn2 is proinflammatory. PMID: 22000474
- These data suggest a possible involvement of urocortin 2 and Ucn 3 in the mechanisms of endometriosis. PMID: 21454316
- Confirm the anti-inflammatory function of VIP, through the modulation of the expression of CRF system that impacts in a reduction of mediators with inflammatory/destructive functions. PMID: 21360527
- Unlike urocortin-1 levels, higher levels of urocortin 2 are not associated with worse left ventricular diastolic performance in patients with chronic systolic heart failure. PMID: 20670842
- Urocortin 2 may play a role in the regulation of estradiol production throughout pregnancy, thereby contributing to the placental regulation of key reproductive events in pregnancy maintenance and parturition. PMID: 19417223
- Placental Ucn2 and Ucn3 expression is sensitive to O(2) tensions and mediated by HIF-1alpha. In preeclampsia, the increased expression of both peptides may reflect a response to the oxidative stress. PMID: 20616367
- SCP and UCN are potent activators of the p42/44 MAPK pathway, with SRP able to induce phosphorylation of p42/44 MAPK as well, albeit not as pronounced PMID: 14519439
- data suggest a distinctive role for corticotropin-releasing hormone receptor 2 specific agonists like urocortin II in the control of myometrial contractility during human pregnancy PMID: 14592950
- data indicate that intracisternal Ucn 2 induced a central CRF(2)-mediated inhibition of gastric emptying involving sympathetic alpha(1)-adrenergic mechanisms independent from the vagus contrasting with the vagal-dependent inhibitory actions of CRF & Ucn 1 PMID: 16223946
- the 38 amino acid urocortin 2 peptide is the minimal pharmacophore PMID: 16476507
- UCN2 and UCN3 are expressed in human placenta, decidua, and fetal membranes and may be proposed as regulators of placental vascular endothelial tone. PMID: 16626608
- Both peptides were immunolocalized in epithelial, stromal, and endothelial cells. PMID: 17074339
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亞細(xì)胞定位：Secreted.
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蛋白家族：Sauvagine/corticotropin-releasing factor/urotensin I family
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