Human Tropomyosin alpha-4 chain(TPM4) ELISA kit

Human Tropomyosin alpha-4 chain(TPM4) ELISA kit

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中文名稱：

人原肌球蛋白α4(TPM4)酶聯(lián)免疫試劑盒
貨號：

CSB-EL024108HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

人原肌球蛋白α4(TPM4)酶聯(lián)免疫試劑盒（CSB-EL024108HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的TPM4含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于基礎(chǔ)科研中TPM4蛋白表達(dá)水平的定量分析，包括但不限于腫瘤細(xì)胞侵襲轉(zhuǎn)移機(jī)制研究、心肌功能異常模型分析、骨骼肌疾病病理探索，以及細(xì)胞骨架動態(tài)變化或相關(guān)信號通路的功能研究。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

epididymis secretory protein Li 108 ELISA Kit; HEL-S-108 ELISA Kit; TM30p1 ELISA Kit; TMP 4 ELISA Kit; TPM4 ELISA Kit; TPM4_HUMAN ELISA Kit; Tropomyosin 4 ELISA Kit; Tropomyosin alpha 4 chain ELISA Kit; Tropomyosin alpha-4 chain ELISA Kit; Tropomyosin-4 ELISA Kit; wu:fb06e07 ELISA Kit; zgc:103436 ELISA Kit; zgc:63909 ELISA Kit
縮寫：

TPM4
Uniprot No.：

P67936
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TPM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-103
1:2	Average %	85
	Range %	81-89
1:4	Average %	91
	Range %	85-97
1:8	Average %	99
	Range %	95-104

回收率：

The recovery of human TPM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-90
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.557	2.597	2.577	2.398
750	1.916	1.936	1.926	1.747
375	1.278	1.298	1.288	1.109
187.5	0.798	0.742	0.770	0.591
94	0.481	0.499	0.490	0.311
47	0.367	0.355	0.361	0.182
23.5	0.264	0.268	0.266	0.087
0	0.178	0.179	0.179

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

BST-1 as a serum protein biomarker involved in neutrophil infiltration in schizophrenia Liang-Jen Wang,The World Journal of Biological Psychiatry,2022
Independent Candidate Serum Protein Biomarkers of Response to Adalimumab and to Infliximab in Rheumatoid Arthritis: An Exploratory Study. Ortea I.et al,PLoS One.,2016

功能：

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
基因功能參考文獻(xiàn)：
1. TPM4 is associated with clinical progression in colon cancer patients and acts as a tumor suppressor in colon cancer cells. PMID: 29455030
2. Tpm4.1 is essential to maintain cell-cell adhesions and to inhibit abnormal increases in cell migration and invasion, which are important to prevent invasion and metastasis of breast cancer cells. PMID: 28431393
3. findings demonstrate a nonredundant role for TPM4 in platelet biogenesis in humans and mice and reveal that truncating variants in TPM4 cause a previously undescribed dominant Mendelian platelet disorder. PMID: 28134622
4. Results show that Tpm4.2 facilitates the transition of NM-2A to the strongly actin-binding myosin-ADP state. PMID: 26847712
5. Data indicate the upregulation of tropomyosin-4 in infiltrating ductal breast carcinomas (IDCAs) tissues, which may suggest its involvement in breast cancer development. PMID: 23812729
6. TPM4, PDIA and SRC8 were also localized to the trophoblast cells, further highlighting the importance of these cytoskeletal remodelling proteins in early pregnancy PMID: 21373848
7. Low TPM4 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
8. Tm4-defined cytoskeleton is a marker of growth and repair/regeneration in response to injury, disease state and stress in skeletal muscle. PMID: 17968984
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Tropomyosin family
組織特異性：

Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets o
數(shù)據(jù)庫鏈接：

HGNC: 12013

OMIM: 600317

KEGG: hsa:7171

UniGene: Hs.631618

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.