Human Thyroxine-Binding Globulin,TBG ELISA Kit

Human Thyroxine-Binding Globulin,TBG ELISA Kit

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文獻引用 (1) 說明書下載

中文名稱：

人甲狀腺結合球蛋白(TBG)酶聯免疫試劑盒
貨號：

CSB-E09501h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人甲狀腺結合球蛋白(TBG)酶聯免疫試劑盒（CSB-E09501h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SERPINA7含量。SERPINA7是一個靶點。它可能在生理病理過程扮演重要角色，目前對其具體背景研究或許與特定疾病相關，研究機制方面可能涉及在體內的信號傳導、與其他分子的相互作用等方面，不過因缺乏更多信息，其確切詳情還待進一步深入探索。試劑盒檢測范圍為12.5 ng/mL-200 ng/mL，適用于實驗室研究甲狀腺功能疾病（如甲亢、甲減、甲狀腺炎）的分子機制、激素代謝調控通路探索，或藥物開發中靶向TBG的作用效果評估本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin A7 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin peptidase inhibitor, clade A, member 7 ELISA Kit; Serpina7 ELISA Kit; T4 binding globulin ELISA Kit; TBG ELISA Kit; TBG, serum ELISA Kit; THBG_HUMAN ELISA Kit; Thyroxin binding globulin ELISA Kit; Thyroxine binding globulin ELISA Kit; Thyroxine-binding globulin ELISA Kit; Thyroxine-binding globulin od serum ELISA Kit
縮寫：

SERPINA7
Uniprot No.：

P05543
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 ng/mL-200 ng/mL
靈敏度：

12.5 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	96
	Range %	92-105
1:4	Average %	84
	Range %	80-89
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of human TBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	96	90-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.121	0.115	0.118
100	0.328	0.328	0.328
50	0.513	0.312	0.413
25	1.015	0.497	0.756
12.5	2.116	2.121	2.119

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Serological proteomic profiling uncovered CDK5RAP2 as a novel marker in benign prostatic hyperplasia H Xie, J Fan, J Wang, T Liu, L Chen, Y Pan, Y Li,Clinical Biochemistry,2024

功能：

Major thyroid hormone transport protein in serum.
基因功能參考文獻：
1. A new SERPINA7 variant associated with thyroxine-binding globulin deficiency in three siblings. PMID: 29733970
2. THBG is a potential plasma biomarker for COPD. PMID: 28579773
3. mutation in a liver-specific enhancer region of the TBG gene caused inherited TBG deficiency. To our knowledge, the present study is the first report of an inherited endocrine disorder caused by a mutation in an enhancer region. PMID: 25361180
4. TBG allosteric regulation is entropy driven. The presence of multiple S states may allow more efficient T4 release due to protease activity. PMID: 23458682
5. the TBG promoter has a role in sustaining transgene expression in liver-specific pattern PMID: 22820390
6. Allosteric modulation of hormone release from thyroxine and corticosteroid-binding globulins. PMID: 21325280
7. Freshly isolated TBG-Chicago exists in loop expelled conformation. At 37C, the protein readily converts to a more stable loop inserted conformation with enhanced heat stability. PMID: 20429632
8. an intragenic A/G polymorphism (125 bp upstream from exon 2) was identified. complete TBG deficiency was homozygous for the polymorphic TBG allele. PMID: 11889160
9. Two novel variants in the thyroxine-binding globulin gene behind the diagnosis of TBG deficiency. homozygous. One involved codon 23 (TCA-->TAA) and the other, codon 223. PMID: 11916615
10. Loop variants of the serpin thyroxine-binding globulin: implications for hormone release upon limited proteolysis. PMID: 11931635
11. guanine deletion at position 1711, codon 201 (Asp) in exon 2 (GAC --> AC) led to a frame shift and premature termination at codon 206, causing a short TBG protein of 205 amino acids (AA) compared to 395 AA of the normal TBG. PMID: 17887925
12. new serpina7 gene variant in three members of the same family results in the replacement of the normal asparagine 233 by isoleucine and, subsequently, in disruption of a glycosylation site PMID: 19415532
13. The first exon (exon 0) is a short noncoding sequence located 1.62 kilobase pairs (kbp) upstream from exon 1. HNF-1 site (located 65 bp upstream of the TSS) is required for the hepatocyte specific expression of the hTBG gene. PMID: 8232304
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亞細胞定位：

Secreted.
蛋白家族：

Serpin family
組織特異性：

Expressed by the liver and secreted in plasma.
數據庫鏈接：

HGNC: 11583

OMIM: 300932

KEGG: hsa:6906

STRING: 9606.ENSP00000329374

UniGene: Hs.76838

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.