Human T-cell surface protein tactile(CD96) ELISA kit

說明書下載

中文名稱：

人T細胞表面觸覺蛋白(CD96)酶聯免疫試劑盒
貨號：

CSB-EL004971HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人T細胞表面觸覺蛋白(CD96)酶聯免疫試劑盒（CSB-EL004971HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CD96含量。CD96是一種重要靶點。它屬于免疫檢查點分子，在腫瘤免疫逃逸等方面有潛在作用。研究機制上，其與配體結合后會影響免疫細胞活性，干擾免疫信號傳導，目前針對CD96開展的研究有望為腫瘤免疫治療開辟新方向。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外實驗中對CD96蛋白表達的動態監測，例如腫瘤微環境研究、免疫細胞功能評估或抗體藥物作用機制探索等領域。本試劑盒為研究CD96在免疫調控網絡中的功能及其與疾病關聯性提供了可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CD96; T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; CD antigen CD96
縮寫：

CD96
Uniprot No.：

P40200
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CD96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	96
	Range %	92-100
1:4	Average %	104
	Range %	100-112
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of human CD96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.045	1.972	2.009	1.836
5	1.502	1.581	1.542	1.369
2.5	1.104	1.054	1.079	0.906
1.25	0.711	0.727	0.719	0.546
0.625	0.510	0.505	0.508	0.335
0.312	0.361	0.349	0.355	0.182
0.156	0.294	0.286	0.290	0.117
0	0.175	0.170	0.173

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
基因功能參考文獻：
1. Lower CD96 expression was also observed in human IL-9(+) compared with IFN-gamma(+) T cells PMID: 29531070
2. Blocking CD96 or TIGIT with mAbs has been shown to improve tumor control in mice. PMID: 27620276
3. CD96 and CD123 are expressed in bone marrow cells of patients with myelodysplastic syndromes PMID: 26642704
4. In the present study we analyzed the expression of four cell surface antigens relevant to human hematopoiesis-CD90, CD96, CD117, and CD123-in bone marrow from pediatric acute myeloid leukemia patients and normal control subjects. PMID: 24751333
5. down-regulation of CD96 is an important aspect of HIV-1 pathogenesis and differential expression is related to cell effector functions and HIV-1 disease course. PMID: 23272144
6. CD96 could serve as a target structure for effector cell-mediated lysis. PMID: 22879978
7. The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, relapse and progression. PMID: 21729528
8. CD96 promotes natural killer (NK) cell adhesion to target cells expressing the poliovirus receptor (PVR), stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. PMID: 15034010
9. CD96 is a cell surface marker present on many acute myeloid leukemia leukemic stem cells PMID: 17576927
10. CD96-driven adhesion to CD155 may be crucial in developmental processes PMID: 19056733
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相關疾病：

C syndrome (CSYN)
亞細胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
數據庫鏈接：

HGNC: 16892

OMIM: 211750

KEGG: hsa:10225

STRING: 9606.ENSP00000283285

UniGene: Hs.142023

Human T-cell surface protein tactile(CD96) ELISA kit

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