Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) ELISA kit

Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) ELISA kit

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中文名稱：

人T細胞免疫球蛋白及粘蛋白域包含蛋白4(TIMD4)酶聯免疫試劑盒
貨號：

CSB-EL023546HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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人T細胞免疫球蛋白及粘蛋白域包含蛋白4(TIMD4)酶聯免疫試劑盒（CSB-EL023546HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TIMD4含量。TIMD4，又稱TIM-4，是一種膜蛋白，作為磷脂酰絲氨酸受體，在免疫應答中發揮作用，如凋亡細胞的吞噬和T細胞調節。TIMD4通過雙模式控制T細胞活化，并促進對凋亡細胞或外源顆粒的吞噬，對T細胞活化和免疫反應至關重要。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于科研領域中對免疫細胞功能調控機制、感染性疾病免疫應答、腫瘤免疫逃逸等方向的研究，為探索TIMD4在病理生理過程中的分子機制提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SMUCKLER ELISA Kit; Spleen; mucin-containing; knockout of lymphotoxin protein ELISA Kit; T cell immunoglobulin and mucin domain containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing molecule ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domains-containing protein 4 ELISA Kit; T-cell membrane protein 4 ELISA Kit; TIM-4 ELISA Kit; Tim4 ELISA Kit; TIMD 4 ELISA Kit; TIMD-4 ELISA Kit; Timd4 ELISA Kit; TIMD4_HUMAN ELISA Kit
縮寫：

TIMD4
Uniprot No.：

Q96H15
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TIMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	99
	Range %	92-102
1:4	Average %	88
	Range %	82-91
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of human TIMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	82-94
EDTA plasma (n=4)	100	94-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.517	2.476	2.497	2.351
5	1.583	1.543	1.563	1.417
2.5	0.951	1.005	0.978	0.832
1.25	0.602	0.637	0.620	0.474
0.625	0.481	0.466	0.474	0.328
0.312	0.291	0.304	0.298	0.152
0.156	0.212	0.217	0.215	0.069
0	0.148	0.144	0.146

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Identification of circulating T‐cell immunoglobulin and mucin domain 4 as a potential biomarker for coronary heart disease M Wang,MedComm,2023
Tim-4 expression increases in ischemic stroke patients and is associated with poor outcome Liyun Xu.et al,J Neuroimmunology,2017
Increased expression of T cell immunoglobulin and mucin domain 4 is positively associated with the disease severity of patients with ankylosing spondylitis Chen D.et al,Inflammation.,2015

功能：

Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
基因功能參考文獻：
1. These findings suggest that the TIMD4-HAVCR1 variants may be the genetic risk factors for coronary heart disease and ischemic stroke PMID: 29208769
2. High expression of TIMD4 in clear cell renal cancer cells was closely related to short progression free survival time, and was associated with resistance to sorafenib. PMID: 28631038
3. VitD deficiency may contribute to the pathogenesis of allergic rhinitis (AR) by increasing the TIM4 expression. The results suggest that to regulate the serum calcitriol levels and the expression of VDR in DCs may be necessary to be taken into account in the treatment of AR. PMID: 28160341
4. This study aimed to investigate the association of two TIM-4 SNPs) with systemic lupus erythematosus (SLE)susceptibility in a Chinese Han population.The results imply that GG genotype of the TIM-4 gene at -1419 site might be associated with the disease activity of SLE. PMID: 28371471
5. Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells PMID: 28740388
6. analysis of the molecular characteristics of both mTIM-4 and hTIM-4 provides a better understanding of the regions of the TIM-4 IgV domain critical for Ebola virus entry PMID: 27122575
7. Tim-4 expression is closely associated with glioma and may have a regulatory role PMID: 26741116
8. Data indicate that T cell immunoglobulin and mucin domain containing 4 (TIM-4) contributes, at least in part, to the pathogenesis of type 2 diabetes mellitus (T2D), possibly by regulating interleukin-1beta (IL-1beta). PMID: 25676395
9. TIM4 binds TIM3 on the surface of polarized Th1 cells to induce Th1 cell apoptosis, which may contribute to the development of Th2-dominant immune disorders. PMID: 26403707
10. TIM-4 rs7700944 and not TIM-1 rs41297579 G>A (-1454) is associated with rheumatoid arthritis(RA) in the present cohort of Egyptian and may be a risk factor for development of RA in Egyptian. PMID: 25899833
11. Data showed up-regulation of TIM-4 in lung cancer tissues and a correlation with poor prognosis. Also, TIM-4 was found to promote growth of lung cancer cells by its interaction with integrin alphavbeta3 through its RGD motif. PMID: 26512878
12. Tim-4 expression on monocytes and Tim-4 level in plasma were more highly increased in ankylosing spondylitis patients than in controls. PMID: 25359708
13. Our results do not support an association between the rs7700944 polymorphism of the TIM-4 gene and rheumatoid arthritis PMID: 24217665
14. Data suggest that genetic polymorphisms in ANGPTL3 (angiopoietin-like 3 protein), TIMD4 (T cell immunoglobulin mucin-4), and apolipoproteins A5 and B are among the genetic determinants of hypertriglyceridemia in Amerindian populations. [REVIEW] PMID: 24768220
15. TIM4 expression is promoted by Cockroach allergen Bla g 7 in dendritic cells leading to Th2 polarization PMID: 24204099
16. TIM-1 and TIM-4 are novel targets for ADAM10- and ADAM17-mediated ectodomain shedding. PMID: 24286866
17. FG-CC' siRNA blocking interaction of Tim-1 and Tim-4 can enhance dendritic cell vaccine activity against gastric cancer. PMID: 22709877
18. There were four SNPs in the promoter region of TIM4 in asthma patients of Chinese Han population, which were in linkage disequilibrium. PMID: 18704309
19. TIM-4 variant or a highly correlated nearby gene of the TIM-4 gene may play a critical role in the pathogenesis of rheumatoid arthritis in many ethnicities. PMID: 22353209
20. macrophage-derived TIM4 plays an important role in the induction of Tregs in gliomas, which may play an important role in tumor tolerance. PMID: 21896488
21. TIM-4 gene polymorphisms are associated with asthma in a Chinese Han population. PMID: 20727045
22. Overexpression of TIM-4 on antigen- presenting cells in transgenic mice reduces the number of antigen-specific T cells that remain after immunization, resulting in reduced secondary T cell responses. PMID: 21037090
23. results showed that Tim-4 mRNA expression in peripheral blood mononuclear cells was significantly higher in system lupus erythematosus patients than in healthy controls, especially those patients in the active phase of disease. PMID: 20140011
24. The polymorphism of 8570G > A in TIM4 may be associated with allergic asthma in the population of Han nationality from Hubei province of China. PMID: 17407086
25. The results demonstrate that Staphylococcus aureus derived Staphylococcal enterotoxin B promotes the TIM4 production in human dendritic cells. PMID: 17439824
26. TIM-4 and TIM-1 are immunologically restricted members of the group of receptors whose recognition of PS is critical for the efficient clearance of apoptotic cells and prevention of autoimmunity. PMID: 18082433
27. Structures of T cell immunoglobulin mucin protein 4 show a metal-Ion-dependent ligand binding site where phosphatidylserine binds. PMID: 18083575
28. Data show that the transmembrane region and cytoplasmic tail of TIM-4 are dispensable for apoptotic cell engulfment, and suggest that TIM-4 is a PtdSer tethering receptor without any direct signaling of its own. PMID: 19217291
29. TIM4 -1419G>A polymorphism might be the genetic factor for the risk of childhood asthma in Chinese Han population. PMID: 19392790
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, TIM family
數據庫鏈接：

HGNC: 25132

OMIM: 610096

KEGG: hsa:91937

STRING: 9606.ENSP00000274532

UniGene: Hs.334907

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.