Human Semenogelin-2(SEMG2) ELISA kit

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產品詳情

產品描述：

人精囊蛋白-2(SEMG2)酶聯免疫試劑盒（CSB-EL021002HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SEMG2含量。人精囊蛋白-2（SEMG2）是一種由精囊腺分泌的蛋白質，主要功能是形成精子周圍的凝膠狀物質，限制精子運動。SEMG2在靈長動物的性別選擇行為中起關鍵作用，其基因的進化速度受性別選擇行為影響，進而影響求偶時的競爭優勢。此外，SEMG2在腫瘤免疫逃逸中也發揮作用，其與多種免疫受體結合，抑制T細胞對腫瘤細胞的殺傷。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，本產品適用于科研領域中對精液蛋白功能、男性生殖生理機制的研究，或通過檢測生物樣本中SEMG2水平探索其與生殖系統疾病的潛在關聯，為體外實驗提供高特異性的定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

SEMG2 ELISA Kit; Semenogelin-2 ELISA Kit; Semenogelin II ELISA Kit; SGII ELISA Kit
縮寫：

SEMG2
Uniprot No.：

Q02383
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human SEMG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	92
	Range %	Sep-87
1:4	Average %	92
	Range %	85-96
1:8	Average %	89
	Range %	87-92

回收率：

The recovery of human SEMG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-96
EDTA plasma (n=4)	103	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Participates in the formation of a gel matrix (sperm coagulum) entrapping the accessory gland secretions and ejaculated spermatozoa.
基因功能參考文獻：
1. SgII transcripts were demonstrated in several tissues, with the strongest signals coming from seminal vesicles, vas deferens, prostate, epididymis and trachea. PMID: 12200457
2. SGII is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036
亞細胞定位：

Secreted.
蛋白家族：

Semenogelin family
組織特異性：

Seminal vesicles, and to a much lesser extent, epididymis.
數據庫鏈接：

HGNC: 10743

OMIM: 182141

KEGG: hsa:6407

STRING: 9606.ENSP00000361855

UniGene: Hs.537218