Human Protein Wnt-5b(WNT5B) ELISA kit
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中文名稱：人Wnt-5b蛋白(WNT5B)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL026139HU
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人Wnt-5b蛋白(WNT5B)酶聯(lián)免疫試劑盒（CSB-EL026139HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的WNT5B含量。WNT5B是一個(gè)靶點(diǎn)。它是Wnt蛋白家族成員，在胚胎發(fā)育、細(xì)胞遷移與極性調(diào)控等多方面發(fā)揮關(guān)鍵作用。其研究機(jī)制涉及與受體結(jié)合激活非經(jīng)典Wnt信號(hào)通路，異常表達(dá)與腫瘤、炎癥等疾病相關(guān)，在臨床研究中受重點(diǎn)關(guān)注。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL，適用于基礎(chǔ)科研領(lǐng)域?qū)NT5B蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，例如癌癥機(jī)制研究、再生醫(yī)學(xué)模型構(gòu)建或信號(hào)通路交互作用分析。科研人員可通過(guò)該產(chǎn)品在體外實(shí)驗(yàn)中探索WNT5B在疾病模型中的功能，或評(píng)估藥物干預(yù)對(duì)相關(guān)信號(hào)通路的調(diào)控效應(yīng)，為分子機(jī)制研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
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別名：MGC2648 ELISA Kit; Protein Wnt-5b ELISA Kit; Wingless type MMTV integration site family, member 5B ELISA Kit; WNT 5B ELISA Kit; WNT 5B protein ELISA Kit; Wnt family member 5B ELISA Kit; Wnt5b ELISA Kit; WNT5B protein ELISA Kit; WNT5B_HUMAN ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Stem Cells
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 87-96 1:2 Average % 98 Range % 94-102 1:4 Average % 94 Range % 89-98 1:8 Average % 85 Range % 80-89 -
回收率：
The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 90-102 EDTA plasma (n=4) 85 80-90 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.663 2.767 2.715 2.616 5 2.326 2.225 2.276 2.177 2.5 1.675 1.578 1.627 1.528 1.25 1.085 1.022 1.054 0.955 0.625 0.507 0.523 0.515 0.416 0.312 0.264 0.276 0.270 0.171 0.156 0.178 0.187 0.183 0.084 0 0.099 0.098 0.099 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Wnt5a induces and maintains prostate cancer cells dormancy in bone Ren D, et al,Journal of Experimental Medicine,2018
- Downregulation of the Canonical WNT Signaling Pathway by TGF尾1 Inhibits Photoreceptor Differentiation of Adult Human M眉ller Glia with Stem Cell Characteristics Angbohang A.et al,Stem Cells Dev.,2016
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靶點(diǎn)詳情
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功能：Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
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基因功能參考文獻(xiàn)：
- exaggerated WNT-5B expression upon cigarette smoke exposure in the bronchial epithelium of COPD patients leads to TGF-beta/Smad3-dependent expression of genes related to airway remodelling PMID: 27126693
- the data suggest that WNT5B induces tube formation by regulating the expression of Snail and Slug proteins through activation of canonical and non-canonical WNT signalling pathways. PMID: 27593938
- Noncanonical Wnt signaling via Wnt5a/5b/11 may have role in the pathogenesis of aortic valve calcification. PMID: 27932350
- WNT-5B induces IL-6 and CXCL8 secretion in pulmonary fibroblasts. PMID: 27036869
- overexpression of WNT5B significantly increased cell proliferation, migration and invasion capacities of the COLO 205 cells in vitro. WNT5B may play an important role in the tumorigenesis of colorectal cancer. PMID: 27121420
- results suggest that Wnt5b-associated exosomes promote cancer cell migration and proliferation in a paracrine manner PMID: 27762090
- these results suggest that TGF-beta1 stimulates HSC-4 cell invasion through the Slug/Wnt-5b/MMP-10 signalling axis. PMID: 26861993
- This study identified WNT5B and CTNNBL1 for peak bone mineral density and body composition in males from the Han Chinese ethnic group. PMID: 26733379
- The interaction between Wnt isoforms and their LRP6 cognate receptor depends on the jutting loop present in Wnt3a but absent in Wnt5b. PMID: 25425204
- Results demonstrate that the Wnt5B pathway may play an important role in atypical teratoid rhabdoid tumor biology. PMID: 25246426
- Report increased Wnt5b levels in adriamycin treated liver cancer stem cells within 4h of treatment. PMID: 24944491
- This study shows that the WNT5B germline variant rs2010851 was significantly identified as a stage-dependent prognostic marker for CC patients after 5-fluorouracil-based adjuvant therapy. PMID: 23817222
- WNT5B is elevated in both in the tumor and the patients' serum from triple negative breast cancer. PMID: 24564888
- Knocking down Wnt5b expression reduced phospho-PKCA levels and cell migration. PMID: 24841200
- Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells. PMID: 24399733
- These results indicate that Wnt5b is involved in the migration ability of OSCC cells through active Cdc42 and RhoA. PMID: 24220306
- WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
- TGF-beta/Smad, integrin/integrin-linked kinase (ILK) and wnt/beta-catenin signaling pathways are involved in epithelial to mesenchymal transition in human renal fibrogenesis. PMID: 24040439
- Differentiation paralleled the activation of Wnt5/Calmodulin signalling by autocrine/paracrine intense secretion of Wnt5a and Wnt5b PMID: 21980498
- This is significantly related to fat cell differentiation, suggesting its possible involvement in the onset of diabetes mellitus type 2 and metabolic syndrome. PMID: 21766608
- WNT5b is upregulated by beta-estradiol in tumor cell lines PMID: 12165812
- WNT5B gene may contribute to conferring susceptibility to type 2 diabetes and may be involved in the pathogenesis of this disease through the regulation of adipocyte function. PMID: 15386214
- In leiomyoma cells, the regulation of Wnt5b expression by retinoids appears to be attenuated. PMID: 15972578
- Variation in WNT5B predisposes to type 2 diabetes in the absence of obesity. PMID: 18555673
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亞細(xì)胞定位：Secreted, extracellular space, extracellular matrix.
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蛋白家族：Wnt family
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