Human Prostaglandin D2(PGD2) ELISA kit

Human Prostaglandin D2(PGD2) ELISA kit

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中文名稱：

人前列腺素D2(PGD2)酶聯免疫試劑盒
貨號：

CSB-E13898h
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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引用文獻
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產品描述：

人前列腺素D2(PGD2)酶聯免疫試劑盒（CSB-E13898h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織培養上清液、組織勻漿、尿液樣本中的PGD2含量。PGD2即前列腺素D2，是一種重要的脂質介質。它參與多種生理病理過程，如炎癥反應、睡眠調節等。在研究機制上，PGD2通過與特定受體結合，激活下游信號通路，調控細胞的功能和基因表達，是炎癥相關疾病研究的熱門靶點。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，該產品可廣泛應用于炎癥性疾病機制研究、藥物篩選模型中的PGD2動態監測、泌尿系統相關科研中的生物標志物分析，以及代謝組學研究中的脂質介質定量檢測，為體外實驗和動物模型研究提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

PGD2
種屬：

Homo sapiens (Human)
樣本類型：

serum, cell culture supernates, tissue homogenates, urine
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-94
1:2	Average %	98
	Range %	91-105
1:4	Average %	99
	Range %	94-110
1:8	Average %	94
	Range %	87-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.985	1.854	1.920	1.840
750	1.403	1.323	1.363	1.283
375	0.912	0.886	0.899	0.819
187.5	0.545	0.525	0.535	0.455
94	0.371	0.359	0.365	0.285
47	0.204	0.198	0.201	0.121
23.5	0.129	0.126	0.128	0.048
0	0.080	0.079	0.080

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Calcitonin gene-related peptide promotes epithelial reparative and anti-colitic functions of IL-4 educated human macrophages BE Callejas, JA Sousa, KL Flannigan,American Journal of Physiology-Gastrointestinal and Liver Physiology,2024
Exploring Long Non-Coding RNAs Associated with IP3/DAG Signaling Pathway as Potential Biomarkers Involved in Mast Cell Degranulation in Chronic Spontaneous Urticaria with 2-Year Follow-Up Y Liang,Journal of Inflammation Research,2022
Cyclooxygenase 1 (COX1) as an indicator of sperm quality in humans Y Liu,Andrologia,2020

■ 常見問題解答

We have previously ordered these kits from you and have been running the project since 2014. I want to keep the same assays as before but I am wondering if you can provide any quality control material? In the past we didn’t have quality control material available. I understand that the kit comes with the required standards but it would be great if a positive and negative control could be purchased to be run with the kit as well. I would purchase these separately if available.Any recommendations as controls for this kit?

Thanks for your inquiry.
CSB-E13898h
The standards in the kit can be used for quantitative analysis and control, so we didn't provide extra control.
Our kit is for research use only and has been optimized. Customers only need to do blank control during the assay and additional quality control is not needed. Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.