Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

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中文名稱：

人前心鈉肽(Pro-ANP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E05149h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人前心鈉肽(Pro-ANP)酶聯(lián)免疫試劑盒（CSB-E05149h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的Pro-ANP含量。Pro-ANP（前心鈉素原）是一個(gè)具有重要生理意義的靶點(diǎn)。它可反映心臟功能狀態(tài)，與心血管系統(tǒng)疾病密切相關(guān)。研究機(jī)制上，主要聚焦于其在調(diào)節(jié)體液平衡、血壓穩(wěn)定等方面的作用，探尋其在疾病發(fā)生發(fā)展中的變化，為相關(guān)疾病治療提供理論依據(jù)。試劑盒檢測(cè)范圍為62.5 pg/mL-4000 pg/mL，該產(chǎn)品廣泛應(yīng)用于心血管疾病機(jī)制研究、藥物干預(yù)效果評(píng)估、動(dòng)物模型心臟功能分析等科研場(chǎng)景，尤其適用于探索心力衰竭、高血壓及相關(guān)代謝性疾病中Pro - ANP的動(dòng)態(tài)變化規(guī)律，為實(shí)驗(yàn)室提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Pro-ANP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	85
	Range %	80-89
1:4	Average %	92
	Range %	87-98
1:8	Average %	104
	Range %	96-108

回收率：

The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	87	82-93

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	1.943	1.896	1.920	1.766
2000	1.452	1.366	1.409	1.255
1000	0.998	0.968	0.983	0.829
500	0.646	0.657	0.652	0.498
250	0.467	0.454	0.461	0.307
125	0.322	0.306	0.314	0.160
62.5	0.209	0.203	0.206	0.052
0	0.155	0.153	0.154

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Evaluation of Cardiac Troponin I and N‐Terminal pro B – Type Natriuretic Peptide Serum Levels in Patients with Alopecia Areata RESM Marie,Clin Exp Dermatol,2020
Associations of rs3918242 and rs2285053 MMP-9 and MMP-2 polymorphisms with the risk, severity, and short- and long-term complications of degenerative mitral valve diseases: a 4.8-year prospective cohort study Balistreri C R.et al,Cardiovascular Pathology,2016

Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit

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