Human Platelet-Derived Growth Factor AA(PDGF-AA) ELISA kit

說明書下載

產品詳情

產品描述：

人血小板衍生生長因子AA(PDGF-AA)酶聯免疫試劑盒（CSB-E17143h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-AA含量。PDGF - AA 即血小板衍生生長因子 AA 型，是一種重要的細胞因子。它在細胞增殖、分化等過程中發揮關鍵作用，與組織修復、血管生成等生理過程相關。研究機制主要圍繞其與受體結合，激活下游信號通路，在腫瘤、纖維化等疾病發病機制中有重要研究價值。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病機制研究、組織再生模型分析、抗纖維化藥物開發等科研領域；為探索 PDGF - AA 在炎癥反應、胚胎發育或創傷愈合中的功能提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PDGF-AA
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-102
1:2	Average %	100
	Range %	92-104
1:4	Average %	92
	Range %	85-96
1:8	Average %	94
	Range %	85-99

回收率：

The recovery of human PDGF-AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	104	95-110

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.