Human Pikachurin(EGFLAM) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人皮卡丘素(EGFLAM)酶聯(lián)免疫試劑盒（CSB-EL007478HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的EGFLAM含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，該產(chǎn)品為科研領(lǐng)域研究者提供高效工具，支持細(xì)胞生物學(xué)實(shí)驗(yàn)中的蛋白表達(dá)動(dòng)態(tài)監(jiān)測、疾病模型中的分子機(jī)制研究，以及生物樣本中EGFLAM的定量分析等應(yīng)用場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Agrin-like protein ELISA Kit; AGRINL ELISA Kit; AGRNL ELISA Kit; EGF-like ELISA Kit; EGF-like; fibronectin type III and laminin G domains ELISA Kit; EGF-like; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; EGFLA_HUMAN ELISA Kit; EGFLAM ELISA Kit; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; FLJ39155 ELISA Kit; Pikachurin ELISA Kit
縮寫：

EGFLAM
Uniprot No.：

Q63HQ2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human EGFLAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	85
	Range %	81-92
1:4	Average %	96
	Range %	90-102
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of human EGFLAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-104
EDTA plasma (n=4)	102	98-106

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.
基因功能參考文獻(xiàn)：
1. Strong candidate gene for macular dystrophy, MCDR3 (human 5p15.33-p13.1). Conclusion is based on a massive expression data set for mouse (103 strains) and joint analysis of RetNet database. PMID: 19727342
亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix. Cell junction, synapse, synaptic cleft. Cell junction, synapse, presynaptic active zone.
數(shù)據(jù)庫鏈接：

HGNC: 26810

OMIM: 617683

KEGG: hsa:133584

STRING: 9606.ENSP00000346964

UniGene: Hs.20103