Human POTE ankyrin domain family member E(POTEE) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

人POTE錨蛋白域家庭成員E(POTEE)酶聯(lián)免疫試劑盒（CSB-EL740863HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的POTEE含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中POTEE蛋白的定量檢測，可用于探索其在腫瘤微環(huán)境中的功能機(jī)制、評估不同病理狀態(tài)下蛋白表達(dá)水平變化，或作為體外模型研究中細(xì)胞信號通路調(diào)控的檢測工具，為腫瘤生物學(xué)、分子病理學(xué)等領(lǐng)域的科研工作提供標(biāo)準(zhǔn)化檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

POTEE ELISA Kit; A26C1A ELISA Kit; POTE2 ELISA Kit; POTE ankyrin domain family member E ELISA Kit; ANKRD26-like family C member 1A ELISA Kit; Prostate ELISA Kit; ovary ELISA Kit; testis-expressed protein on chromosome 2 ELISA Kit; POTE-2 ELISA Kit
縮寫：

POTEE
Uniprot No.：

Q6S8J3
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human POTEE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	98
	Range %	93-103
1:4	Average %	102
	Range %	98-106
1:8	Average %	91
	Range %	86-97

回收率：

The recovery of human POTEE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-108
EDTA plasma (n=4)	89	83-94

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.