Human Neuropeptide-like protein C4orf48(C4orf48) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人神經肽樣蛋白C4orf48(C4orf48)酶聯免疫試劑盒（CSB-EL003989HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4orf48含量。C4orf48是一種位于人類4號染色體上的基因，編碼一種神經肽樣蛋白。該蛋白在神經系統的發育中表達，可能與智力障礙和神經退行性疾病有關。近期研究發現，C4orf48通過RNA結合機制促進腎臟纖維化，成為慢性腎臟病治療的新靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎研究領域探索C4orf48在神經系統發育、神經退行性疾病模型中的表達調控，或結合組織樣本分析其區域性分布特征。科研人員可通過檢測體液或組織裂解液中的蛋白含量，研究其在特定生理病理過程中的潛在作用機制，為神經生物學研究提供實驗工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C4orf48 ELISA Kit; Chr4_55 ELISA Kit; Neuropeptide-like protein C4orf48 ELISA Kit
縮寫：

C4orf48
Uniprot No.：

Q5BLP8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cell Biology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4orf48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	87-98
1:8	Average %	103
	Range %	97-108

回收率：

The recovery of human C4orf48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	81-92
EDTA plasma (n=4)	102	97-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.