Human N-Acetyl-Ser-Asp-Lys-Pro,AcSDKP ELISA Kit

Human N-Acetyl-Ser-Asp-Lys-Pro,AcSDKP ELISA Kit

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中文名稱：

人N-乙酰基-絲氨酰-天門冬酰-賴氨酰-脯氨酸(AcSDKP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08949h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人N-乙酰基-絲氨酰-天門冬酰-賴氨酰-脯氨酸(AcSDKP)酶聯(lián)免疫試劑盒（CSB-E08949h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的AcSDKP含量。AcSDKP 是一個(gè)重要靶點(diǎn)。它在人體生理活動(dòng)中有著一定作用，其背景涉及在細(xì)胞調(diào)節(jié)等方面的關(guān)聯(lián)。研究機(jī)制聚焦于它與相關(guān)生理過(guò)程的相互作用，如對(duì)細(xì)胞增殖、分化的影響，在醫(yī)學(xué)研究中有望為疾病治療尤其是一些細(xì)胞異常疾病提供新方向。試劑盒檢測(cè)范圍為0.235 ng/mL-15 ng/mL，該產(chǎn)品可廣泛應(yīng)用于基礎(chǔ)研究領(lǐng)域，如體外探究AcSDKP在纖維化疾病模型中的動(dòng)態(tài)表達(dá)、評(píng)估藥物干預(yù)對(duì)肽段水平的影響，或結(jié)合動(dòng)物實(shí)驗(yàn)分析其在組織修復(fù)、干細(xì)胞調(diào)控等生理過(guò)程中的作用機(jī)制，為相關(guān)科研課題提供可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
縮寫：

AcSDKP
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.235 ng/mL-15 ng/mL
靈敏度：

0.058 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AcSDKP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	100
	Range %	95-106
1:4	Average %	93
	Range %	87-97
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of human AcSDKP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-108
EDTA plasma (n=4)	89	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
15	2.465	2.346	2.406	2.246
7.5	2.139	2.055	2.097	1.937
3.75	1.812	1.746	1.779	1.619
1.88	1.437	1.524	1.481	1.321
0.94	0.962	0.971	0.967	0.807
0.47	0.554	0.566	0.560	0.400
0.235	0.316	0.297	0.307	0.147
0	0.158	0.161	0.160

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

N-Acetyl-Seryl-Asparyl-Lysyl-Proline interaction with ACE2-Ang (1-7)-Mas axis attenuates pulmonary fibrosis in silicotic rats Xuemin G, et al,Experimental Physiology,2019

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.