Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

Human Mitochondrial import inner membrane translocase subunit TIM14(DNAJC19) ELISA kit

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中文名稱：

人線粒體進口內膜轉位酶亞基TIM14(DNAJC19)酶聯免疫試劑盒
貨號：

CSB-EL007026HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人線粒體進口內膜轉位酶亞基TIM14(DNAJC19)酶聯免疫試劑盒（CSB-EL007026HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的DNAJC19含量。DNAJC19是一種基因，作為治療非小細胞肺癌的靶點。研究發現，該基因參與NSCLC細胞的增殖和遷移，通過抑制其表達可以有效降低腫瘤細胞的侵襲、遷移和生長能力，成為NSCLC患者的潛在治療靶標。試劑盒檢測范圍為28 pg/mL-1800 pg/mL，通過高特異性抗體對靶蛋白進行精準捕獲與信號放大，可為線粒體蛋白質轉運機制研究、疾病模型中線粒體功能障礙評估以及藥物干預效果分析提供可靠工具，廣泛應用于神經退行性疾病、心血管疾病等科研領域的分子機制探索及生物標志物定量分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

DnaJ (Hsp40) homolog; subfamily C; member 19 ELISA Kit; DnaJ homolog subfamily C member 19 ELISA Kit; DNAJC19 ELISA Kit; homolog of yeast TIM14 ELISA Kit; Mitochondrial import inner membrane translocase subunit TIM14 ELISA Kit; PAM18 ELISA Kit; TIM 14 ELISA Kit; TIM14 ELISA Kit; TIM14_HUMAN ELISA Kit; TIMM 14 ELISA Kit; TIMM14 ELISA Kit; translocase of the inner mitochondrial membrane 14 ELISA Kit; Translocase of the inner mitochondrial membrane 14; yeast homolog of ELISA Kit
縮寫：

DNAJC19
Uniprot No.：

Q96DA6
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

28 pg/mL-1800 pg/mL
靈敏度：

7 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DNAJC19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	86
	Range %	81-90
1:4	Average %	93
	Range %	89-98
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of human DNAJC19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-107
EDTA plasma (n=4)	95	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1800	2.656	2.719	2.688	2.579
900	2.287	2.172	2.230	2.121
450	1.653	1.707	1.680	1.571
225	1.135	1.071	1.103	0.994
112.5	0.681	0.653	0.667	0.558
56	0.374	0.362	0.368	0.259
28	0.257	0.252	0.255	0.146
0	0.112	0.105	0.109

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Mitochondrial co-chaperone which forms a complex with prohibitins to regulate cardiolipin remodeling. May be a component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity.
基因功能參考文獻：
1. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. PMID: 27330077
2. The loss of DNAJC19/PHB complexes affects cardiolipin acylation and leads to the accumulation of cardiolipin species with altered acyl chains. PMID: 24856930
3. A report of a new mutation in the human DNAJC19 gene that causes early onset dilated cardiomyopathy syndrome in two brothers of Finnish origin. PMID: 22797137
4. The association of dilated cardiomyopathy with ataxia (DCMA) syndrome with a segment of 3q26.33 and the identification of a splice mutation in a novel gene DNAJC19 in DCMA patients. PMID: 16055927
5. Dilated cardiomyopathy caused by homozygous mutations in a novel gene, DNAJC19, presumed to play a role in importation of mitochondrial proteins. PMID: 17244376
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相關疾病：

3-methylglutaconic aciduria 5 (MGA5)
亞細胞定位：

Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
蛋白家族：

TIM14 family
組織特異性：

Ubiquitously expressed.
數據庫鏈接：

HGNC: 30528

OMIM: 608977

KEGG: hsa:131118

STRING: 9606.ENSP00000372005

UniGene: Hs.230601

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.