Human Ly6/PLAUR domain-containing protein 1(LYPD1) ELISA kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人Ly6/PLAUR結構域蛋白1(LYPD1)酶聯免疫試劑盒（CSB-EL013261HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的LYPD1含量。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎研究領域，可用于體外評估不同生理或病理條件下（如癌癥模型、神經退行性疾病模型）生物樣本中LYPD1的表達水平變化，為探索其分子機制及功能研究提供定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FLJ41033 ELISA Kit; LY6/PLAUR domain containing 1 ELISA Kit; Ly6/PLAUR domain-containing protein 1 ELISA Kit; Lypd1 ELISA Kit; LYPD1_HUMAN ELISA Kit; LYPDC1 ELISA Kit; MGC29643 ELISA Kit; PHTS ELISA Kit; Putative HeLa tumor suppressor ELISA Kit
縮寫：

LYPD1
Uniprot No.：

Q8N2G4
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-91
1:2	Average %	97
	Range %	92-104
1:4	Average %	85
	Range %	82-90
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of human LYPD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. May play a role in the intracellular trafficking of alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their expression at the cell surface. May be involved in the control of anxiety.
基因功能參考文獻：
1. The PHTS is involved in HeLaHF reversion independently of the p53 pathway, and Expression profiling revealed that PHTS is one of the genes that is up-regulated in HeLaHF but not in HeLa. PMID: 16413018
亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor.
數據庫鏈接：

HGNC: 28431

OMIM: 610450

KEGG: hsa:116372

STRING: 9606.ENSP00000380605

UniGene: Hs.432395