Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit
產(chǎn)品詳情
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產(chǎn)品描述：
人肝表達(dá)抗菌肽2(LEAP2)酶聯(lián)免疫試劑盒（CSB-EL012853HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的LEAP2含量。人肝表達(dá)抗菌肽2（LEAP2）是一種新型活性多肽，具有抑菌和調(diào)節(jié)體重功能。其結(jié)構(gòu)穩(wěn)定，在先天免疫系統(tǒng)中發(fā)揮關(guān)鍵作用，能抵御細(xì)菌感染，并參與調(diào)節(jié)食欲、能量代謝等生理過(guò)程。LEAP2通過(guò)破壞細(xì)菌細(xì)胞膜和調(diào)節(jié)免疫細(xì)胞功能來(lái)發(fā)揮其抗菌和免疫調(diào)節(jié)作用。試劑盒檢測(cè)范圍為0.312 ng/mL-20 ng/mL，支持科研人員探索LEAP2在代謝性疾病模型中的動(dòng)態(tài)變化、評(píng)估肝臟功能狀態(tài)，或研究其與微生物感染相關(guān)的免疫調(diào)節(jié)機(jī)制。為肝臟代謝研究、免疫調(diào)控機(jī)制探索及藥物開(kāi)發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：
LEAP2Liver-expressed antimicrobial peptide 2 ELISA Kit; LEAP-2 ELISA Kit
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縮寫(xiě)：
LEAP2
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測(cè)范圍：
0.312 ng/mL-20 ng/mL
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靈敏度：
0.078 ng/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測(cè)波長(zhǎng)：
450 nm
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研究領(lǐng)域：
Cardiovascular
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測(cè)定原理：
quantitative
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測(cè)定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LEAP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 105 | | |
| Range % | 98-111 | | |
| 1:2 | Average % | 99 | | |
| Range % | 90-104 | | |
| 1:4 | Average % | 97 | | |
| Range % | 86-103 | | |
| 1:8 | Average % | 103 | | |
| Range % | 95-107 | | |
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回收率：
| The recovery of human LEAP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 99 | 91-104 | | |
| EDTA plasma (n=4) | 102 | 95-107 | | |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 20 | 2.512 | 2.600 | 2.556 | 2.403 | | | | 10 | 2.101 | 1.978 | 2.040 | 1.887 | | | | 5 | 1.403 | 1.415 | 1.409 | 1.256 | | | | 2.5 | 0.824 | 0.844 | 0.834 | 0.681 | | | | 1.25 | 0.489 | 0.501 | 0.495 | 0.342 | | | | 0.625 | 0.311 | 0.323 | 0.317 | 0.164 | | | | 0.312 | 0.231 | 0.226 | 0.229 | 0.076 | | | | 0 | 0.156 | 0.150 | 0.153 | | | |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LEAP2 antibody.
- Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated LEAP2 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
- Substrate A (1 x 7 ml)
- Substrate B (1 x 7 ml)
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
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