Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

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中文名稱：

人角蛋白I型細胞骨架23(KRT23)酶聯免疫試劑盒
貨號：

CSB-EL012539HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人角蛋白I型細胞骨架23(KRT23)酶聯免疫試劑盒（CSB-EL012539HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KRT23含量。KRT23 作為一個靶點，目前可能在醫學等領域具有一定研究價值。它屬于角蛋白相關靶點。相關研究機制或許圍繞其在細胞生長、分化、代謝等過程中的作用展開，探索其在疾病發生發展中的角色，以尋求可能的治療干預靶點。試劑盒檢測范圍為0.9 ng/mL-60 ng/mL，適用于基礎醫學研究中KRT23蛋白表達水平的動態監測，例如肝纖維化病理機制探索、腫瘤微環境分析或藥物干預效果評估等科研場景；可用于體外檢測細胞培養上清、動物模型或人體組織樣本中的KRT23含量，為相關疾病的分子機制研究提供實驗工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CK 23 ELISA Kit; CK-23 ELISA Kit; CK23 ELISA Kit; Cytokeratin 23 ELISA Kit; Cytokeratin-23 ELISA Kit; Cytokeratin23 ELISA Kit; DKFZP434G032 ELISA Kit; HAIK 1 ELISA Kit; HAIK1 ELISA Kit; Histone deacetylase inducible keratin 23 ELISA Kit; Hyperacetylation inducible type I keratin ELISA Kit; K1C23_HUMAN ELISA Kit; K23 ELISA Kit; Keratin 23 (histone deacetylase inducible) ELISA Kit; Keratin 23 ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 23 ELISA Kit; Keratin-23 ELISA Kit; Keratin23 ELISA Kit; KRT 23 ELISA Kit; KRT23 ELISA Kit; MGC26158 ELISA Kit; type I cytoskeletal 23 ELISA Kit; Type I intermediate filament cytokeratin ELISA Kit
縮寫：

KRT23
Uniprot No.：

Q9C075
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.23 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human KRT23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	91
	Range %	87-96
1:4	Average %	88
	Range %	82-94
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of human KRT23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	93	87-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.761	2.562	2.662	2.537
30	2.155	2.062	2.109	1.984
15	1.337	1.305	1.321	1.196
7.5	0.745	0.720	0.733	0.608
3.75	0.413	0.405	0.409	0.284
1.8	0.276	0.283	0.280	0.155
0.9	0.193	0.189	0.191	0.066
0	0.125	0.124	0.125

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.