- A 96-well Assay plate --The 96-well plate has been pre-coated with HEV antigen
- Negative Control (1 x 0.4ml) --Eliminate false positive
- Positive Control (1 x 0.4ml) --Used to evaluate the validity, stability, and comparability of experimental results.
- HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HEV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (1x 12ml) --Dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
- Substrate A (1 x 6ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
- Substrate B (1 x 6ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
- Stop Solution (1 x 6ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
- An Instruction manual
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