Human Glypican-4(GPC4) ELISA kit

Human Glypican-4(GPC4) ELISA kit

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中文名稱：

人磷脂酰肌醇聚糖4(GPC4)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL009706HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

人磷脂酰肌醇聚糖4(GPC4)酶聯(lián)免疫試劑盒（CSB-EL009706HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的GPC4含量。GPC4，全稱Glypican 4，是一種位于Xq26.2染色體上的基因，其編碼的蛋白質(zhì)屬于細(xì)胞表面硫酸乙酰肝素蛋白聚糖家族。該基因在細(xì)胞分裂和生長(zhǎng)調(diào)節(jié)中發(fā)揮作用，可能與Simpson-Golabi-Behmel綜合征有關(guān)。研究機(jī)制方面，GPC4基因的表達(dá)與多種生物學(xué)過(guò)程相關(guān)，包括細(xì)胞信號(hào)傳導(dǎo)、細(xì)胞增殖和腫瘤發(fā)生等。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL，適用于基礎(chǔ)科研領(lǐng)域中對(duì)GPC4蛋白的功能機(jī)制研究，例如腫瘤微環(huán)境分析、干細(xì)胞特性探索、胚胎發(fā)育相關(guān)信號(hào)通路研究等場(chǎng)景，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit
縮寫：

GPC4
Uniprot No.：

O75487
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	92
	Range %	88-96
1:4	Average %	87
	Range %	81-94
1:8	Average %	98
	Range %	92-106

回收率：

The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	95	91-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.254	2.355	2.305	2.163
20	1.428	1.528	1.478	1.336
10	0.935	0.956	0.946	0.804
5	0.556	0.567	0.562	0.420
2.5	0.372	0.361	0.367	0.225
1.25	0.268	0.257	0.263	0.121
0.625	0.215	0.212	0.214	0.072
0	0.142	0.141	0.142

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
基因功能參考文獻(xiàn)：
1. The T allele of GPC4 may represent a risk factor for Epstein-Barr virus-associated gastric carcinoma. PMID: 27071854
2. According to behavioral studies, downregulation of Gpc4 by Gpc4 siRNA decreased spontaneous seizure frequency, while upregulation of Gpc4 by recombinant Gpc4 overexpression led to a converse result. These findings support the hypothesis that increased expression of Gpc4 in the brain is associated with epileptic seizures. PMID: 27425250
3. serum level elevated in polycystic ovary syndrome and correlated with fat distribution and cardiovascular risk PMID: 26486309
4. Data suggest gender difference in circulating GPC4 levels in nonalcoholic fatty liver disease; GPC4 levels in women appear to correlate with cardiometabolic risk factors (adiposity/body fat distribution, insulin resistance, and arterial stiffness). PMID: 23633195
5. new function of miR-125a by targeting gene glypican-4 in cell growth process PMID: 22644326
6. Findings establish that Gpc4 acts at the interface of extrinsic and intrinsic signal regulation to fine tune stem cell fate. PMID: 22761013
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亞細(xì)胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.; [Secreted glypican-4]: Secreted, extracellular space.
蛋白家族：

Glypican family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 4452

OMIM: 300168

KEGG: hsa:2239

STRING: 9606.ENSP00000359864

UniGene: Hs.58367

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.