Human Fibulin-7(FBLN7) ELISA kit

說明書下載

產品詳情

產品描述：

人腓骨蛋白-7(FBLN7)酶聯免疫試劑盒（CSB-EL008455HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FBLN7含量。FBLN7，即纖連蛋白7，是一種胞外基質蛋白，參與多種蛋白間的相互作用。研究發現，FBLN7在心血管發育、牙齒發育、腫瘤轉移等過程中發揮重要作用。其研究機制涉及調節細胞粘附、生長、遷移和存活等細胞功能。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于基礎科研中FBLN7的蛋白表達分析，例如探究其在組織修復、胚胎發育或特定病理模型（如纖維化疾病、腫瘤微環境）中的動態變化，為體外實驗或動物模型研究提供高效工具。通過兼容多種生物樣本類型，研究者可靈活應用于體液檢測或組織水平的分子機制探索，為細胞外基質相關研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

FBLN7 ELISA Kit; FBLN7_HUMAN ELISA Kit; FIBL-7 ELISA Kit; Fibl7 ELISA Kit; Fibulin-7 ELISA Kit; TM14 ELISA Kit
縮寫：

FBLN7
Uniprot No.：

Q53RD9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-99
1:2	Average %	95
	Range %	87-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	97
	Range %	91-101

回收率：

The recovery of human FBLN7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	91	84-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

An adhesion molecule that interacts with extracellular matrix molecules in developing teeth and may play important roles in differentiation and maintenance of odontoblasts as well as in dentin formation.
基因功能參考文獻：
1. Fbln7-C terminal fragment inhibited the HUVEC tube formation and the vessel sprouting in aortic ring assays. PMID: 24480309
2. Dysregulation of fibulin expression by anti-Ro/SSA antibodiess may contribute to disorganization of the extracellular environment and thus cause injury to the salivary gland architecture and functionality observed in Sjogren syndrome PMID: 19229767
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibulin family
數據庫鏈接：

HGNC: 26740

OMIM: 611551

KEGG: hsa:129804

STRING: 9606.ENSP00000331411

UniGene: Hs.437696