Human Fibrinogen(FB) ELISA Kit

Human Fibrinogen(FB) ELISA Kit

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文獻引用 (8) 說明書下載

中文名稱：

人纖維蛋白原（Fb）酶聯(lián)免疫試劑盒
貨號：

CSB-E13656h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

人纖維蛋白原（Fb）酶聯(lián)免疫試劑盒（CSB-E13656h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Fb含量。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究中凝血/纖溶系統(tǒng)調(diào)控機制探索、肝臟功能評估、心血管疾病動物模型驗證，以及炎癥相關(guān)病理過程的分子機制研究，為體外藥物篩選、疾病模型構(gòu)建和生物標志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

Fb
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 μg/mL-20 μg/mL
靈敏度：

0.078 μg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-92
1:200	Average %	98
	Range %	90-105
1:400	Average %	97
	Range %	90-105
1:800	Average %	93
	Range %	86-98

回收率：

The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	98	90-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average	Corrected
20	2.648	2.701	2.675	2.583
10	2.159	2.169	2.164	2.072
5	1.476	1.536	1.506	1.414
2.5	0.864	0.876	0.870	0.778
1.25	0.443	0.475	0.459	0.367
0.625	0.234	0.253	0.244	0.152
0.312	0.146	0.154	0.150	0.058
0	0.095	0.089	0.092

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Calcium Chloride vs. Mechanical Preparation of Fibrinogen-Depleted Human Platelet Lysate: Implications for Umbilical Cord Mesenchymal Stem Cell Culture YT Lim, M Barathan, YL Tan, YT Lee, JX Law,Life,2024
Preparation of Fibrinogen-Depleted Human Platelet Lysate to Support Heparin-Free Expansion of Umbilical Cord-Derived Mesenchymal Stem Cells LT Kee,Journal of translational medicine,2023
Identification and characterization of novel mutations in Chinese patients with congenital fibrinogen disorders P Zhou,Blood Cells, Molecules, and Diseases,2020
Lower level of complement component C3 and C3a in the plasma means poor outcome in the patients with hepatitis B virus related acute-on-chronic liver … Q Li,BMC Gastroenterol,2020
Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury Islam D, et al,American journal of respiratory and critical care medicine,2018
A novel fibrinogen gamma-chain mutation, p. Cys165Arg, causes disruption of the γ165Cys-Bβ227Cys disulfide bond and ultimately leads to hypofibrinogenemia WJ Zhou, et al,Thrombosis Research,2018
Two symptomatic cases of dysfibrinogenemia in China: one with gamma-chain Arg275Cys mutation and another without detectable mutation in fibrinogen genes. Bin Q et al,Blood Coagul Fibrinolysis,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.