Human Dipeptidase 1(DPEP1) ELISA kit

Human Dipeptidase 1(DPEP1) ELISA kit

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中文名稱：

人二肽酶1(DPEP1)酶聯免疫試劑盒
貨號：

CSB-EL007123HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人二肽酶1(DPEP1)酶聯免疫試劑盒（CSB-EL007123HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的DPEP1含量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，科研人員可通過該產品探究DPEP1在疾病發生發展中的分子機制，評估藥物干預對DPEP1活性的影響，或進行不同組織/細胞模型中蛋白表達差異的比較研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Dehydropeptidase-I ELISA Kit; Dipeptidase 1 (renal) ELISA Kit; Dipeptidase 1 ELISA Kit; DPEP1 ELISA Kit; DPEP1_HUMAN ELISA Kit; hRDP ELISA Kit; MBD1 ELISA Kit; MDP ELISA Kit; Microsomal dipeptidase ELISA Kit; RDP ELISA Kit; Renal dipeptidase ELISA Kit
縮寫：

DPEP1
Uniprot No.：

P16444
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.86 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human DPEP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-93
1:2	Average %	97
	Range %	91-106
1:4	Average %	102
	Range %	92-111
1:8	Average %	94
	Range %	86-99

回收率：

The recovery of human DPEP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	1.885	1.752	1.819	1.739
750	1.303	1.223	1.263	1.183
375	0.812	0.778	0.795	0.715
187.5	0.465	0.453	0.459	0.379
94	0.331	0.319	0.325	0.245
47	0.194	0.191	0.193	0.113
23.5	0.129	0.126	0.128	0.048
0	0.080	0.079	0.080

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4. Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation. Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics.; Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver.
基因功能參考文獻：
1. The results suggest that DPEP1 promotes cancer metastasis by regulating E-cadherin plasticity and that it might be a potential therapeutic target for preventing the progression of colon cancer. PMID: 26824987
2. In this study, we present an analysis of Neanderthal introgression at the dipeptidase 1 gene, DPEP1. PMID: 26392408
3. Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification. PMID: 23839495
4. DPEP1 plays a role in pancreatic cancer aggressiveness. PMID: 22363658
5. DPEP1 is expressed in the early stages of colon carcinogenesis and affects cancer cell invasiveness. PMID: 20824289
6. we identified a novel immunohistochemical marker, dipeptidase 1, to distinguish primary mucinous ovarian cancers from ovarian metastasis of colorectal cancers. PMID: 21076463
7. Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis PMID: 12144777
8. DPEP1 has a role in colorectal carcinoma PMID: 15145522
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亞細胞定位：

Apical cell membrane; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI-anchor.
蛋白家族：

Metallo-dependent hydrolases superfamily, Peptidase M19 family
組織特異性：

Expressed in lung and kidneys.
數據庫鏈接：

HGNC: 3002

OMIM: 179780

KEGG: hsa:1800

STRING: 9606.ENSP00000261615

UniGene: Hs.109

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.