Human Complement 4,C4 ELISA Kit
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中文名稱：
人補體蛋白4(C4)酶聯免疫試劑盒
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貨號：
CSB-E08705h
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規格：
96T/48T
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價格：
￥3200/￥2500
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其他：
產品詳情
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產品描述：
人補體蛋白4(C4)酶聯免疫試劑盒（CSB-E08705h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的C4含量。試劑盒檢測范圍為0.41 ng/mL-300 ng/mL，靈敏度為0.41 ng/mL。適用于基礎醫學研究中C4蛋白的動態監測，例如感染性疾病模型的補體激活機制分析、自身免疫性疾病動物模型的病理機制探索，或藥物干預下補體通路的功能研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
Acidic C4 ELISA Kit; Acidic complement C4 ELISA Kit; Basic C4 ELISA Kit; Basic complement C4 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4; Chido form ELISA Kit; C4; Rodgers from ELISA Kit; C4A anaphylatoxin ELISA Kit; C4A ELISA Kit; C4A2 ELISA Kit; C4A3 ELISA Kit; C4A4 ELISA Kit; C4A6 ELISA Kit; C4AD ELISA Kit; C4B ELISA Kit; C4B_2 ELISA Kit; C4B1 ELISA Kit; C4B12 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; C4BD ELISA Kit; C4F ELISA Kit; C4S ELISA Kit; CH ELISA Kit; Chido form of C4 ELISA Kit; CO4 ELISA Kit; CO4A_HUMAN ELISA Kit; Complement C4 A ELISA Kit; Complement C4 B ELISA Kit; Complement C4 gamma chain ELISA Kit; complement C4-A ELISA Kit; complement C4-B ELISA Kit; complement C4-B-like ELISA Kit; complement C4B1a ELISA Kit; Complement component 4A (Rodgers blood group) ELISA Kit; Complement component 4A ELISA Kit; complement component 4B (Chido blood group) ELISA Kit; complement component 4B (Chido blood group); copy 2 ELISA Kit; Complement component 4B (Childo blood group) ELISA Kit; Complement component 4B ELISA Kit; Complement component 4F ELISA Kit; Complement component 4S ELISA Kit; CPAMD2 ELISA Kit; CPAMD3 ELISA Kit; RG ELISA Kit; Rodgers form of C4 ELISA Kit
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縮寫：
C4
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
0.41 ng/mL-300 ng/mL
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靈敏度：
0.41 ng/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Others
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
96 |
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| Range % |
93-99 |
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| 1:2000 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:4000 |
Average % |
89 |
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| Range % |
86-92 |
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| 1:8000 |
Average % |
100 |
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| Range % |
97-104 |
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回收率：
| The recovery of human C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
86 |
83-90 |
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| EDTA plasma (n=4) |
95 |
91-99 |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.873 |
2.897 |
2.885 |
2.786 |
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| 100 |
1.916 |
1.968 |
1.942 |
1.843 |
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| 33.3 |
1.281 |
1.299 |
1.290 |
1.191 |
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| 11.1 |
0.878 |
0.898 |
0.888 |
0.789 |
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| 3.7 |
0.495 |
0.472 |
0.484 |
0.385 |
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| 1.23 |
0.195 |
0.212 |
0.204 |
0.105 |
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| 0.41 |
0.187 |
0.192 |
0.190 |
0.091 |
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| 0 |
0.101 |
0.097 |
0.099 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human C4 antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated C4 antibody (100 x concentrate) (120 μl/bottle) ---Bind to the C4 in the samples or standards and react with the substrate to make the solution chromogenic.
- One HRP-conjugate Diluent(20 ml/bottle) ---Dilute the HRP-conjugated C4 antibody
- Two vials Sample Diluent (20 ml/bottle) ---Dilute the sample solution.
- One vial Wash Buffer (25x concentrate) (20ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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Diagnosis of Rheumatic and Autoimmune Diseases Dataset
MF Mahdi, A Jahani, DH Abd,Data in Brief,2025
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Potential biomarkers of spinal dural arteriovenous fistula: C4BPA and C1QA
Y Wang,Journal of neuroinflammation,2022
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Lower level of complement component C3 and C3a in the plasma means poor outcome in the patients with hepatitis B virus related acute-on-chronic liver …
Q Li,BMC Gastroenterol,2020
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Copy number variations of complement component C4 are associated with behcet's disease but not with ankylosing spondylitis associated with Acute anterior uveitis
Hou S et al,Arthritis Rheum,2013
靶點詳情
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最新研究進展：
C4（補體C4）是補體系統中的一種蛋白質，是補體系統經典途徑和凝集素途徑的共同組成部分。C4具有多種生物學作用，包括誘導炎癥反應、增強免疫細胞對病原體的吞噬和殺傷能力等。C4水平的異常可與多種自身免疫疾病相關，如系統性紅斑狼瘡等。
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功能：
Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
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基因功能參考文獻：
- The purpose of this study was to evaluate C4A and C4B in patients with congenital adrenal hyperplasia in relation to CYP21A2 genotype and psychiatric and autoimmune comorbidity. We determined the copy numbers of C4A and C4B in 145 patients with CAH .No association was found between C4 copy number and autoimmune disease. PMID: 30465166
- Low C4 in systemic lupus erythematosus patients is due to consumption rather than deficient synthesis related to lower C4A & B gene copy numbers. PMID: 30041577
- Report strong association of systemic lupus erythematosus in individuals with low copy numbers of C4 and in particular in patients with complete deficiency of C4A. PMID: 29050534
- An increase in serum C4, as wall as a decrease in C3, was an important outcome determinant for patients with immunoglobulin A nephropathy. PMID: 28697742
- for the first time, a complete overview of C4 in SLE from genetic variation to binding capacity using a novel test. As this test detects crossing over of Rodgers and Chido antigens, it will allow for more accurate measurement of C4 in future studies. PMID: 29080553
- The study re-evaluates low-resolution crystal structures of C4 via interactive molecular-dynamics flexible fitting. In terms of biology, the results provide a better structural framework for understanding the pivotal function of the C4 protein within the complement system. PMID: 27599733
- An elevated number of C4 genes was observed in Alzheimer's disease (AD) patients as compared with healthy controls. The presence of high C4A and C4B copy numbers in AD patients could explain the increased C4 protein expression observed in AD patients, thus highlighting a possible role for C4A and C4B copy number variations in the risk of developing AD. PMID: 27758680
- This study shows that the C4c/C4 ratio seems to be a better diagnostic measure than total antigenic C4 alone. Our findings underline that screening with total antigenic C4 implies a risk of overlooking C1-INH-HAE patients. PMID: 28412283
- In comparison with C4-intact patients, C4-deficient patients had a different clinical/serologic lupus-like phenotype and lacked the lupus interferon signature. PMID: 27274010
- C4 copy number variations and deficiency of C4A both play an important role in the risk and manifestations of systemic lupus erythematosus in East Asian and European populations PMID: 26814708
- Complement C4A deficiency appears to be an important factor for the genetic risk and pathogenesis of juvenile dermatomyositis, particularly in patients with a DR3-positive background. PMID: 26493816
- Coronary atherosclerosis is distinguished by serum C4 complement up-regulation and ceruloplasmin down-regulation. PMID: 28091899
- find strong statistical significance for association of increased copy number of C4A (OR 0.81 (0.73; 0.89);P = 4.4 x 10(-5)), with the effect most pronounced in individuals over 78 years (OR 0.67 (0.55; 0.81)) and females PMID: 27090374
- Copy Number Variation Scan Identifies Complement Component C4 as Novel Susceptibility Gene for Crohn's Disease. PMID: 26595553
- genetic polymorphism is associated with acute graft versus host disease in unrelated hematopoietic stem cell transplantation PMID: 26602146
- important role of complement C4a in inhibiting the HBV DNA secretion in chronic hepatitis b PMID: 26119402
- C4A and C4B gene copy numbers are stronger risk factors for juvenile-onset than for adult-onset systemic lupus erythematosus. PMID: 26800705
- Complement C4A deficiency (gene copy number <=1) was identified as a risk factor in a case-control study of juvenile dermatomyositis, particularly when subjects concurrently carried the HLA-DRB1*0301 allele. PMID: 26493816
- Increased age, rs2857009 single nucleotide polymorphism of complement component C4 and hepatitis C virus genotype were associated with disease progression. PMID: 25573496
- Low C4 was associated with non-Hodgkin's lymphoma in primary Sjogren's syndrome. PMID: 26359802
- Schizophrenia risk from complex variation of complement component 4 PMID: 26814963
- C4 levels were significantly lower in Factor XII-hereditary angioedema than in idiopathic non-histaminergic acquired angioedema. PMID: 25744496
- our study indicates that Finnish NTM patients had significantly more often C4 deficiencies than the healthy control subjects. PMID: 24638111
- Complement components C3a and C4a, but not C5a, display antimicrobial activity against P. aeruginosa, E. coli, B. subtilis, and C. albicans. PMID: 17132627
- C4 is a novel cellular substrate of the HCV NS3/4A protease. PMID: 24349192
- Low C4 gene copy numbers are associated with superior graft survival in patients transplanted with a deceased donor kidney. PMID: 23715124
- serum C4a desArg is a potential biomarker for the severity of histological findings in patients with IgA nephropathy. PMID: 23708385
- Our findings indicate that a high copy number of C4A confers risk for Behcet disease by modulating the expression of C4A and enhancing IL-6 production. PMID: 23918728
- Studies indicate that initiation of lectin compleme pathway leads to activation of the serine proteases MASP-1 and MASP-2 resulting in deposition of C4 on the activator and assembly of the C3 convertase. PMID: 23911397
- C4d might be a biomarker for evaluating the risk for IUGR and disease control in patients with systemic lupus erythematosus and pregnancy-induced hypertension. PMID: 23530559
- Studies indicate beta-defensins (DEFB4, DEFB103, DEFB104), chemokine ligand 3 like 1 (CCL3L1), Fc gamma receptor 3B (FCGR3B), and complement component C4 (C4) for copy number variation in disease association. PMID: 22837109
- Complement 4a plasma protein was identified as increased in Alzheimer's disease PMID: 22052466
- Deletion variants of C4 were found to be associated with SLE in Korean women, but did not reach statistical significance. PMID: 23335107
- study concludes that the association of C4 gene copy with systemic lupus erythematosus(SLE)was replicated in Chinese Han population, which highlighted the importance of C4 in SLE pathogenesis of diverse populations PMID: 21904924
- The precise order and size of all C4 genes were determined in RCCX, a multiallelic copy number variation locus. PMID: 22785613
- C4A deficiency is one of the minor defects of the innate immunity that may predispose children and young adults to recurrent respiratory infections. PMID: 22406254
- congenital adrenal hyperplasia patients have increased C4 copy number variation, with mutation-specific associations that may be protective for autoimmune disease PMID: 22886582
- C4A appears to associate with the protection of residual beta-cell function in new-onset type 1 diabetes. PMID: 22151770
- analysis of gene copy number of complement C4A, C4B and C4A silencing mutation by real-time quantitative polymerase chain reaction PMID: 22737222
- analysis of the structural basis for activation of the complement system by component C4 cleavage PMID: 22949645
- Complement C4a gene expression is regulated both by obesity and by the region between visceral and subcutaneous adipose tissue. PMID: 22616691
- Past, present, and future perspectives of C4d as a biomarker, focusing on its use in solid organ transplantation and discussing its possible new roles in autoimmunity and pregnancy. Review. PMID: 22297669
- We showed no evidence for a role of hs-CRP, C3 and C4 in the association between BMI and asthma symptoms in overweight children. PMID: 21801245
- Although complete homozygous deficiency of complement C4 is one of the strongest genetic risk factors for SLE, partial C4 deficiency states do not independently predispose to the disease. PMID: 22387014
- Individuals with 4, 2, and 2 copies of C4, C4A and C4B genes, especially those with A2B2 polymorphism may associate with the development of Graves' disease PMID: 21943165
- Data show that in the UK cohort, total C4 GCN ranged from 2 to 6, with copy numbers from 0 to 4 observed for both C4A and C4B, while in the Spanish cohort, C4A GCN from 0 to 6 and C4B GCN from 0 to 5. PMID: 21857912
- The study shows the complement component C4A in the plasmas of sePE women is lower than the severe, late-onset PE women, and the Apolipoprotein A-I level is higher in sePE women than slPE women. PMID: 21677994
- C4 mRNA levels of the two isoforms (C4A and C4B) were significantly reduced in hepatocytes transfected with RNA from HCV genotype 1a or 2a. PMID: 21345967
- The reduction in olfactory function in these hereditary angioedema cases seems to correlate with complement C4 and CH50 levels. PMID: 20649895
- not demonstrate that C4 gene copy number associates with transplant outcome PMID: 21164027
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相關疾病：
Complement component 4A deficiency (C4AD); Systemic lupus erythematosus (SLE)
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亞細胞定位：
Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
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組織特異性：
Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extr
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