Human Calpain-5(CAPN5) ELISA kit

Human Calpain-5(CAPN5) ELISA kit

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中文名稱：

人鈣蛋白酶5(CAPN5)酶聯(lián)免疫試劑盒
貨號：

CSB-EL004498HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人鈣蛋白酶5(CAPN5)酶聯(lián)免疫試劑盒（CSB-EL004498HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CAPN5含量。CAPN5（鈣蛋白酶5）是一種鈣依賴性半胱氨酸蛋白酶，參與細胞信號傳導和多種細胞過程。其突變與新生血管性炎性玻璃體視網膜病變有關，研究其功能有助于理解相關疾病的發(fā)生機制。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于體外培養(yǎng)細胞模型、動物組織樣本或體液標本的CAPN5蛋白定量分析，為研究其在疾病發(fā)生發(fā)展中的作用、藥物干預效果評估或分子通路機制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

ADNIV ELISA Kit; Calpain htra 3 ELISA Kit; Calpain htra-3 ELISA Kit; Calpain-5 ELISA Kit; CAN5_HUMAN ELISA Kit; CAPN 5 ELISA Kit; Capn5 ELISA Kit; nCL 3 ELISA Kit; nCL-3 ELISA Kit; New calpain 3 ELISA Kit; VRNI ELISA Kit
縮寫：

CAPN5
Uniprot No.：

O15484
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CAPN5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-91
1:2	Average %	105
	Range %	99-108
1:4	Average %	89
	Range %	85-93
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of human CAPN5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	95	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.972	1.917	1.945	1.787
1000	1.339	1.358	1.349	1.191
500	0.979	0.956	0.968	0.810
250	0.728	0.712	0.720	0.562
125	0.448	0.454	0.451	0.293
62.5	0.381	0.369	0.375	0.217
31.25	0.261	0.277	0.269	0.111
0	0.160	0.156	0.158

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Calcium-regulated non-lysosomal thiol-protease.
基因功能參考文獻：
1. The relative domain rotation of 60-100 degrees we found for mini-calpain-5 (a non-classical calpain) is significantly greater than the largest rotation previously observed for a classical calpain PMID: 27474374
2. CAPN5 localization at the photoreceptor synapse and with mitochondria explains the neural circuitry phenotype in human CAPN5 disease alleles. PMID: 27152965
3. A novel CAPN5 (c.750G>T, p.Lys250Asn) missense mutation causes uveitis and neovascular retinal detachment. PMID: 25856303
4. CAPN5 mutation in hereditary uveitis: the R243L mutation increases calpain catalytic activity and triggers intraocular inflammation PMID: 25994508
5. CAPN5 expression can be suppressed by shRNA-based RNA interference PMID: 25216694
6. autosomal dominant neovascular inflammatory vitreoretinopathy is due to CAPN5 gain-of-function rather than haploinsufficiency. PMID: 24381307
7. Calpain-5 mutations cause autoimmune uveitis, retinal neovascularization, and photoreceptor degeneration. PMID: 23055945
8. Results describe the cloning and characterization of rat calpain-5, which is highly homologous to human and mouse sequences. PMID: 15464980
9. CAPN5 polymorphisms are associated with a risk of polycystic ovary syndrome. PMID: 16396936
10. CAPN5 seems to influence traits related to increased risk for cardiovascular diseases and play a role as a candidate gene for metabolic syndrome PMID: 17227582
11. We have found significant interaction between CAPN5 and PPARD genes that reduces risk for obesity in 55%. CAPN5 and PPARD gene products may also interact in vivo. PMID: 18657264
12. Data examine possible allelic imbalance in papillary thyroid cancer at EMSY, CAPN5, and PAK1, as candidate genes within 11q13.5-q14 region using a single nucleotide polymorphism-based analysis. PMID: 18787380
13. Calpain5 was expressed in endometrial stromal and glandular cells throughout the menstrual cycle and in decidua, and its expression was decreased in both stromal and glandular cells from women with endometriosis compared with that of fertile controls. PMID: 18829447
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相關疾病：

Vitreoretinopathy, neovascular inflammatory (VRNI)
蛋白家族：

Peptidase C2 family
組織特異性：

Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
數(shù)據(jù)庫鏈接：

HGNC: 1482

OMIM: 193235

KEGG: hsa:726

STRING: 9606.ENSP00000278559

UniGene: Hs.248153

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.