Human Bradykinin,BK ELISA Kit

產(chǎn)品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人血管舒緩激肽(BK)酶聯(lián)免疫試劑盒（CSB-E09155h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BK含量。試劑盒檢測范圍為0.312 pg/mL-20 pg/mL，適用于心血管疾病機制研究、炎癥相關信號通路探索、藥物干預效果評估等科研領域；為研究BK在血管內皮功能調控、過敏性休克或局部水腫等病理模型中的作用提供有效工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

BK
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 pg/mL-20 pg/mL
靈敏度：

0.078 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	90-95
1:200	Average %	103
	Range %	98-107
1:400	Average %	93
	Range %	86-97
1:800	Average %	97
	Range %	91-103

回收率：

The recovery of human BK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	97	93-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.