Human Beta-klotho(KLB) ELISA kit
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中文名稱：人β-克洛素(KLB)酶聯(lián)免疫試劑盒
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貨號：CSB-EL012378HU
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規(guī)格：96T/48T
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價格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人β-克洛素(KLB)酶聯(lián)免疫試劑盒（CSB-EL012378HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的KLB含量。KLB（Klotho beta）是一種蛋白質(zhì)編碼基因，參與RET信號通路和HIV生命周期。其在多種代謝過程中起調(diào)節(jié)作用，但在癌癥中的作用尚不明確。研究發(fā)現(xiàn)，βKlotho在肝癌組織中的表達(dá)下調(diào)，且其重新引入肝癌細(xì)胞可抑制其增殖。KLB的研究機(jī)制涉及FGF19/21-KLB-FGFRs信號通路，該通路在脂質(zhì)代謝和抗肥胖中發(fā)揮作用。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，適用于體外實驗中對KLB蛋白表達(dá)量的動態(tài)監(jiān)測，如探索其在肥胖、糖尿病、非酒精性脂肪肝等代謝相關(guān)疾病中的分子機(jī)制，或用于藥物篩選模型中評估化合物對KLB表達(dá)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Beta-klotho ELISA Kit; BetaKlotho ELISA Kit; BKL ELISA Kit; KLB ELISA Kit; KLOTB_HUMAN ELISA Kit; Klotho beta like protein ELISA Kit; Klotho beta-like protein ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates, cell lysates
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檢測范圍：62.5 pg/mL-4000 pg/mL
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靈敏度：15.6 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Metabolism
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human KLB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 102 Range % 96-105 1:2 Average % 88 Range % 84-92 1:4 Average % 94 Range % 89-98 1:8 Average % 92 Range % 88-94 -
回收率：
The recovery of human KLB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 102 96-106 EDTA plasma (n=4) 88 82-92 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 4000 2.372 2.278 2.325 2.223 2000 1.746 1.844 1.795 1.693 1000 1.396 1.293 1.345 1.243 500 0.972 0.961 0.967 0.865 250 0.588 0.576 0.582 0.480 125 0.354 0.368 0.361 0.259 62.5 0.225 0.230 0.228 0.126 0 0.103 0.101 0.102 -
數(shù)據(jù)處理：
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貨期：3-5 working days
產(chǎn)品評價
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靶點詳情
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功能：Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
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基因功能參考文獻(xiàn)：
- Knockdown of beta-Klotho produced the opposite effects. In conclusion, beta-Klotho inhibits EMT and plays a tumorsuppressive role in prostate cancer (PCa) , linking FGF/FGFR/beta-Klotho signaling to the regulation of PCa progression. PMID: 29749458
- crystal structures of free and ligand-bound beta-klotho extracellular regions that reveal the molecular mechanism that underlies the specificity of FGF21 towards beta-klotho and demonstrate how the FGFR is activated in a klotho-dependent manner PMID: 29342135
- the metabolic FGF21/KLB/FGFR1 pathway is involved in congenital hypogonadotropic hypogonadism (CHH) Genetic screening of 334 CHH patients identified seven heterozygous loss-of-function KLB mutations in 13 patients (4%). Most patients with KLB mutations (9/13) exhibited metabolic defects. PMID: 28754744
- We conducted a genome-wide association meta-analysis and replication study among >105,000 individuals of European ancestry and identified beta-Klotho (KLB) as a locus associated with alcohol consumption (rs11940694; P = 9.2 x 10(-12)). PMID: 27911795
- A high expression level of KLbeta, but not KLalpha, was an independent predictive factor of short progression free survival for non-muscle invasive bladder cancer PMID: 27573985
- Our results suggest that KLbeta plays important roles in tumor invasion and progression, and its concentration may be a valuable urinebased marker for the detection of bladder cancer. PMID: 27573985
- obesity appears as the predominant determinant of the abnormalities in FGF21 and FGF19 levels. Opposite changes in beta-Klotho expression in fat and liver indicate potential tissue-specific alterations in the responsiveness to endocrine FGFs in obesity PMID: 24813368
- ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
- Variants in genes involved in feedback regulation of bile acid synthesis (KLB, P=0.06 and FGFR4, P=0.09) were potentially associated with the irritable bowel syndrome-diarrhea subgroup with elevated serum C4. PMID: 25070056
- In-depth DNA sequencing identified additional genetic coding and noncoding variants in KLB that are associated with fecal bile acids excretion or colonic transit in Irritable bowel syndrome-diarrhea. PMID: 24200957
- betaKlotho suppresses tumor growth in hepatocellular carcinoma by regulating Akt/GSK-3beta/cyclin D1 signaling pathway. PMID: 23383245
- KLB and FGFR1 form a 1:1 heterocomplex independent of the galectin lattice that transitions to a 1:2 complex upon the addition of FGF21. PMID: 22523080
- KLB-silencing in Huh7 cells decreased cell proliferation and suppressed FGFR4 downstream signaling. PMID: 22439738
- Differential specificity of endocrine FGF19 and FGF21 to FGFR1 and FGFR4 in complex with KLB. PMID: 22442730
- Deletion of the D1 and the D1-D2 linker (the D1/linker region) from FGFR1c led to beta-Klotho-independent receptor activation by FGF21, suggesting that there may be a direct interaction between FGF21 and the D1/linker region-deficient FGFR1c. PMID: 22248288
- Polymorphism KLB rs4975017 may influence the colonic transit response to colesevelam in female patients with irritable bowel syndrome with diarrhea. PMID: 22271411
- Data suggest that KLB is an important regulator in the immortalization of HCECs by facilitating FGF19 growth factor signaling. PMID: 22020932
- Sulfated glycosaminoglycans are required for specific and sensitive fibroblast growth factor (FGF) 19 signaling via FGF receptor 4 and betaKlotho. PMID: 21653700
- A functional KLB gene variant mediating protein stability associates with colonic transit in irritable bowel syndrome with diarrhea. This association is modulated by 2 genetic variants in FGFR4. PMID: 21396369
- Metabolic regulator betaKlotho interacts with fibroblast growth factor receptor 4 (FGFR4) to induce apoptosis and inhibit tumor cell proliferation. PMID: 20657013
- The down-regulation of the renal klotho gene could increase renal damage induced by angiotensin II, while klotho gene induction could have therapeutic possibilities in treating angiotensin II-induced kidney damage. PMID: 16358222
- Liver-specific activities of FGF19 require Klotho beta. PMID: 17627937
- a functional FGF19 receptor may consist of FGF receptor (FGFR) and heparan sulfate complexed with either alphaKlotho or betaKlotho PMID: 17711860
- High expression of Klotho is associated with ovarian disease progression PMID: 18259951
- Both FGF23 and FGF21 require intact alpha or betaKlotho for signaling, respectively, whereas FGF19 can signal through a Klotho chimera consisting of the N terminus of alphaKlotho and the C terminus of betaKlotho. PMID: 18829467
- These data demonstrate that the C-terminus of FGF21 is critical for binding to beta-Klotho and the N-terminus is critical for fibroblast growth factor receptor (FGFR) activation. PMID: 19059246
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亞細(xì)胞定位：Cell membrane; Single-pass type III membrane protein.
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蛋白家族：Glycosyl hydrolase 1 family, Klotho subfamily
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