Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

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中文名稱：

人ATP合成酶脂質結合蛋白，線粒體(ATP5G1)酶聯(lián)免疫試劑盒
貨號：

CSB-EL002359HU
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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人ATP合成酶脂質結合蛋白，線粒體(ATP5G1)酶聯(lián)免疫試劑盒（CSB-EL002359HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的ATP5G1含量。ATP5G1是線粒體ATP合成酶的一個亞基，參與細胞能量代謝。其研究機制涉及線粒體ATP合成酶的組裝和功能，通過氧化磷酸化過程合成ATP，為細胞提供能量。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于基礎科研領域對能量代謝機制的研究，可用于探索細胞代謝異常、神經(jīng)退行性疾病模型中線粒體功能障礙、心血管疾病能量代謝調控，以及衰老相關線粒體活性變化等課題。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ATP5MC1; ATP5G1; ATP synthase F(0 complex subunit C1, mitochondrial; ATP synthase lipid-binding protein; ATP synthase membrane subunit c locus 1; ATP synthase proteolipid P1; ATP synthase proton-transporting mitochondrial F(0 complex subunit C1; ATPase protein 9; ATPase subunit c
縮寫：

ATP5G1
Uniprot No.：

P05496
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.86 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ATP5G1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	94
	Range %	89-98
1:4	Average %	89
	Range %	85-93
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of human ATP5G1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	94	90-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.637	2.612	2.625	2.480
750	2.215	2.118	2.167	2.022
375	1.697	1.683	1.690	1.545
187.5	1.107	1.118	1.113	0.968
94	0.611	0.621	0.616	0.471
47	0.365	0.372	0.369	0.224
23.5	0.242	0.231	0.237	0.092
0	0.146	0.144	0.145

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Fo ATP synthase C subunit serum levels in patients with ST-segment Elevation Myocardial Infarction: Preliminary findings Campo G.et al,Int J Cardiol.,2016

功能：

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. A homomeric c-ring of probably 10 subunits is part of the complex rotary element.
基因功能參考文獻：
1. ATP5G1, ATP5G2, and ATP5G3 of the ATP synthase are not involved in forming the permeability transition pore. PMID: 28289229
2. ATP5G1 turnover increases upon depletion of ZC3H14, double knockdown of ZC3H14 and the nonsense-mediated decay factor, UPF1, rescues ATP5G1 transcript levels. Furthermore, fractionation reveals an increase in the amount of ATP5G1 pre-mRNA that reaches the cytoplasm when ZC3H14 is depleted and that ZC3H14 binds to ATP5G1 pre-mRNA in the nucleus. PMID: 27563065
3. The subunit c isoforms are nonredundant, because they differ functionally by their targeting peptides, which, in addition to mediating mitochondrial protein import, play a yet undiscovered role in respiratory chain maintenance. PMID: 19889836
亞細胞定位：

Mitochondrion membrane; Multi-pass membrane protein.
蛋白家族：

ATPase C chain family
數(shù)據(jù)庫鏈接：

HGNC: 841

OMIM: 603192

KEGG: hsa:516

STRING: 9606.ENSP00000348205

UniGene: Hs.80986

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.