Human A-kinase anchor protein 4(AKAP4) ELISA kit

Human A-kinase anchor protein 4(AKAP4) ELISA kit

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中文名稱：

人蛋白激酶A錨定蛋白(AKAP4)酶聯免疫試劑盒
貨號：

CSB-EL001526HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

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產品描述：

人蛋白激酶A錨定蛋白(AKAP4)酶聯免疫試劑盒（CSB-EL001526HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AKAP4含量。AKAP4是一種與精子鞭毛功能相關的蛋白，其缺失或減弱會導致精子活力降低，進而引發不育。研究顯示，AKAP4在精子鞭毛的纖維鞘和運動調控中起關鍵作用，其表達異常與多種男性不育癥相關。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，該產品適用于生殖系統發育、生殖細胞功能調控及相關疾病機制的基礎研究，例如利用動物模型探索不育癥或睪丸發育異常的分子機制，為科研人員提供穩定、特異的AKAP4定量分析工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

A kinase (PRKA) anchor protein 4 ELISA Kit; A kinase anchor protein 4 ELISA Kit; A kinase anchor protein 82 kDa ELISA Kit; A-kinase anchor protein 4 ELISA Kit; A-kinase anchor protein 82 kDa ELISA Kit; AKAP 4 ELISA Kit; AKAP 82 ELISA Kit; AKAP-4 ELISA Kit; AKAP4 ELISA Kit; AKAP4_HUMAN ELISA Kit; AKAP82 ELISA Kit; FSC 1 ELISA Kit; FSC1 ELISA Kit; hAKAP 82 ELISA Kit; hAKAP82 ELISA Kit; HI ELISA Kit; Major sperm fibrous sheath protein ELISA Kit; p82 ELISA Kit; PRKA 4 ELISA Kit; PRKA4 ELISA Kit; Protein kinase A anchoring protein 4 ELISA Kit; Protein kinase A-anchoring protein 4 ELISA Kit; Testis specific gene HI ELISA Kit
縮寫：

AKAP4
Uniprot No.：

Q5JQC9
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

25 pg/mL-1600 pg/mL
靈敏度：

6.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	89
	Range %	85-96
1:4	Average %	105
	Range %	100-114
1:8	Average %	85
	Range %	80-89

回收率：

The recovery of human AKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-87

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.339	2.257	2.298	2.159
800	1.813	1.676	1.745	1.606
400	1.156	1.124	1.140	1.001
200	0.699	0.743	0.721	0.582
100	0.387	0.415	0.401	0.262
50	0.297	0.304	0.301	0.162
25	0.215	0.209	0.212	0.073
0	0.137	0.141	0.139

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
基因功能參考文獻：
1. High AKAP4 expression is associated with reduced sperm motility. PMID: 29581387
2. A significant association was found between AKAP4 gene expression and metastasis (P-value: 0.045), expression of the CTAG1B (NY-ESO-1) gene was not observed in our cases. PMID: 29480665
3. the physiological role of the negative crosstalk between the cAMP/PKA/AKAP4 and the PKC/ERK1/2 pathways is to regulate capacitation and acrosome reaction. PMID: 27901058
4. AKAP4 role in the proliferation and metastasis of thyroid cancer.AKAP4 is highly expressed in thyroid cancer. PMID: 27983916
5. AKAP4 appears to be a novel Colorectal cancer-associated antigen with a potential for developing as a new clinical therapeutic target PMID: 26590805
6. AKAP4 is a highly accurate biomarker for the detection of early stage lung cancer. PMID: 26160834
7. SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients. PMID: 25739119
8. The putative role of AKAP4 in early tumorigenesis is implicated as a biomarker and immunotherapeutic target for cervical cancer. PMID: 23478221
9. Ablation of AKAP4 protein caused significant inhibition in cellular proliferation, colony-forming ability, migration and invasion ability of tumor cells. PMID: 23764900
10. data suggests that AKAP4 may be used as serum based diagnostic test for an early detection and diagnosis of breast cancer and may be a potential target for immunotherapeutic use PMID: 23451156
11. We demonstrate the aberrant expression of AKAP-4 in prostate cancer, which will potentially be developed as a biomarker in prostate cancer. PMID: 21520158
12. AKAP4 is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036
13. Role of AKAP4 in sperm motility is unclear, vut absent or weak AKAP4-labelling is associated with absent or weak sperm. motility. PMID: 17712481
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亞細胞定位：

Cell projection, cilium, flagellum. Note=Localizes to the principle piece of the sperm flagellum.
蛋白家族：

AKAP110 family
組織特異性：

Testis specific; only expressed in round spermatids.
數據庫鏈接：

HGNC: 374

OMIM: 300185

KEGG: hsa:8852

STRING: 9606.ENSP00000351327

UniGene: Hs.97633

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.