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中文名稱:人6-羥基硫酸褪黑素(6HMS)酶聯免疫試劑盒
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貨號:CSB-EQ027870HU
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人6-羥基硫酸褪黑素(6HMS)酶聯免疫試劑盒(CSB-EQ027870HU)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的6HMS含量。人6-羥基硫酸褪黑素(6HMS)是褪黑素的代謝產物,參與調節生物節律和抗氧化作用。研究發現,6HMS在妊娠期高血壓疾病(HDCP)中發揮重要作用,通過調節胎盤氧化應激水平和內皮功能,參與HDCP的發病機制。試劑盒檢測范圍為10 pg/mL- 400 pg/mL,可應用于褪黑素代謝通路研究、神經內分泌系統功能分析,或探索6HMS在睡眠障礙、神經退行性疾病及免疫調節中的潛在作用機制,為體外實驗提供穩定可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:6HMS
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:10 pg/mL- 400 pg/mL
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靈敏度:10 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human 6HMS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 92-98 1:2 Average % 95 Range % 87-105 1:4 Average % 96 Range % 89-102 1:8 Average % 92 Range % 88-96 -
回收率:
The recovery of human 6HMS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 89-100 EDTA plasma (n=4) 95 91-101 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 400 0.154 0.156 0.155 200 0.360 0.342 0.351 92 0.572 0.543 0.558 30 0.856 0.860 0.858 10 1.475 1.489 1.482 0 2.341 2.435 2.388 -
數據處理:
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貨期:3-5 working days
引用文獻
- Modulation of aqueous humor melatonin levels by yellow-filter and its protective effect on lens VE Lledó,Journal of Photochemistry and Photobiology B: Biology,2021
- Melatonin as a potential therapy for sepsis: a phase I dose escalation study and an ex vivo whole blood model under conditions of sepsis Galley HF et al,J Pineal Res,2014
相關問答
I would like to know if you recommend using lysis buffer in order to remove the blood from the tissue when running the 6-hydroxymelatonin ELISA KIT in tissue. Our specimens are largely supplied by blood what could mask results.
So sorry that we have no experience on using lysis buffer remove the blood from the tissue.
Anyway,if it helps,we are glad to recommend to you our sample method on tissue stated it as in the manual"100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C"
And if you worry the blood effect, pls use PBS to wash the sample longer.
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