Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

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中文名稱：

人2,3-二磷酸甘油酸(2,3-DPG)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E09861h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人2,3-二磷酸甘油酸(2,3-DPG)酶聯(lián)免疫試劑盒（CSB-E09861h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的2,3DPG含量。2,3-DPG即2,3-二磷酸甘油酸，是紅細(xì)胞糖酵解支路的中間產(chǎn)物。它能調(diào)節(jié)血紅蛋白對(duì)氧氣的親和力，降低其與氧的結(jié)合力，促進(jìn)氧氣釋放。在貧血、高原適應(yīng)等過(guò)程中發(fā)揮重要作用。當(dāng)前對(duì)其在相關(guān)疾病機(jī)制及治療靶點(diǎn)方面有一定研究。試劑盒檢測(cè)范圍為0.312 μmol/mL-20 μmol/mL，適用于基礎(chǔ)研究中紅細(xì)胞代謝功能評(píng)估、缺氧生理機(jī)制探索以及血液系統(tǒng)疾病模型的機(jī)制分析，例如貧血、高原反應(yīng)或心血管相關(guān)疾病的體外實(shí)驗(yàn)研究，滿足細(xì)胞能量代謝、運(yùn)動(dòng)生理學(xué)或病理狀態(tài)下的氧運(yùn)輸調(diào)控等科研需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

2,3DPG
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測(cè)范圍：

0.312 μmol/mL-20 μmol/mL
靈敏度：

0.078 μmol/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
Range %	92-100
1:2	Average %	98
Range %	92-103
1:4	Average %	95
Range %	90-99
1:8	Average %	90
Range %	85-96

回收率：

The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	84	80-89

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µmol/ml	OD1	OD2	Average	Corrected
20	2.873	2.712	2.793	2.636
10	2.265	2.149	2.207	2.050
5	1.372	1.328	1.350	1.193
2.5	0.854	0.813	0.834	0.677
1.25	0.457	0.481	0.469	0.312
0.625	0.337	0.357	0.347	0.190
0.312	0.202	0.207	0.205	0.048
0	0.161	0.152	0.157

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

AVALIA??O DA EFICáCIA DE SUPLEMENTA??O DE CONCENTRADOS DE HEMáCIAS PARA FINS TRANSFUSIONAIS: ESTUDOS DE VIABILIDADE CELULAR E PAR?METROS BIOQUíMICOS LNG Carvalho, FF Costa, MT Mesquita,Hematology, Transfusion and Cell Therapy,2024
Photodynamic inactivation of antibiotic-resistant bacteria in whole blood using riboflavin photodynamic method L Zhu,Frontiers in microbiology,2024
Modification of Biochemical Derangements and VEGF Secretion may Prevent Diabetic Retinopathy (DR) Mondal L K, et al,Journal of Clinical & Experimental Ophthalmology,2019
Temporary impact of blood donation on physical performance and hematologic variables in women Stangerup I.et al,Transfusion. ,2017
In vitro efficacy of frozen erythrocytes: implementation of new strategic blood stores to alleviate resource shortage (issue revisited). Cetinkaya RA.et al,Turk J Med Sci.,2015

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.