Human β-defensins 2 ELISA Kit
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中文名稱：
人β-防御素2酶聯免疫試劑盒
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貨號：
CSB-E13201h
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規格：
96T/48T
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價格：
￥3600/￥2500
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其他：
產品詳情
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產品描述：
人β-防御素2酶聯免疫試劑盒（CSB-E13201h）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養上清液、組織勻漿樣本中的DEFB4含量。DEFB4即β-防御素 4，是一種內源性抗菌肽。它在機體固有免疫中起關鍵作用，具有廣譜抗菌活性，能抵御細菌、真菌等病原體。其研究機制主要聚焦在它與病原體膜相互作用、免疫調節功能及信號通路激活等方面，為抗感染治療提供新思路。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品適用于科研領域對感染性病原體應答機制、慢性炎癥性疾病（如銀屑病、特應性皮炎）的病理研究，也可用于評估藥物或生物制劑對 hBD - 2 分泌的調控作用，為免疫調節、黏膜屏障功能及感染免疫方向的體外實驗提供可靠工具。樣本兼容性廣泛，支持多種生物樣本類型的穩定檢測，操作流程標準化，適用于實驗室常規科研需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：
BD 2 ELISA Kit; BD-2 ELISA Kit; BD2 ELISA Kit; beta 2 ELISA Kit; beta 2 Defensin ELISA Kit; Beta defensin 2 ELISA Kit; Beta defensin 4B ELISA Kit; Beta-defensin 2 ELISA Kit; Beta-defensin 4A ELISA Kit; DEF B2 ELISA Kit; DEF B4 ELISA Kit; DEFB 102 ELISA Kit; DEFB 2 ELISA Kit; DEFB 4 ELISA Kit; DEFB102 ELISA Kit; DEFB2 ELISA Kit; DEFB2; formerly ELISA Kit; DEFB4 ELISA Kit; DEFB4; formerly ELISA Kit; DEFB4B ELISA Kit; DEFB4P ELISA Kit; Defensin ELISA Kit; Defensin beta 2 ELISA Kit; Defensin; beta 4 ELISA Kit; Defensin; beta 4; pseudogene ELISA Kit; Defensin; beta 4A ELISA Kit; Defensin; beta 4B ELISA Kit; Defensin; beta; 2; formerly ELISA Kit; Defensin; beta; 4; formerly ELISA Kit; DFB4A_HUMAN ELISA Kit; HBD 2 ELISA Kit; hBD-2 ELISA Kit; HBD2 ELISA Kit; mBD-2 ELISA Kit; SAP 1 ELISA Kit; SAP1 ELISA Kit; Skin antimicrobial peptide 1 ELISA Kit; Skin-antimicrobial peptide 1 ELISA Kit
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縮寫：
DEFB4
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, urine, cell culture supernates, tissue homogenates
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檢測范圍：
62.5 pg/mL-4000 pg/mL
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靈敏度：
15.6 pg/mL
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Microbiology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 81-92 | |
| 1:2 | Average % | 94 | |
| Range % | 90-100 | |
| 1:4 | Average % | 98 | |
| Range % | 91-110 | |
| 1:8 | Average % | 91 | |
| Range % | 85-98 | |
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回收率：
| The recovery of human β-defensins 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 89-98 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | pg/ml | OD1 | OD2 | Average | Corrected | | | 4000 | 2.821 | 2.752 | 2.787 | 2.689 | | | 2000 | 2.182 | 2.076 | 2.129 | 2.031 | | | 1000 | 1.438 | 1.364 | 1.401 | 1.303 | | | 500 | 0.802 | 0.793 | 0.798 | 0.700 | | | 250 | 0.471 | 0.437 | 0.454 | 0.356 | | | 125 | 0.302 | 0.290 | 0.296 | 0.198 | | | 62.5 | 0.243 | 0.229 | 0.236 | 0.138 | | | 0 | 0.099 | 0.097 | 0.098 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DEFB4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled DEFB4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數據處理：
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貨期：
3-5 working days
引用文獻
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The Role of Beta-Defensin 2 in Preventing Preterm Birth with Chorioamnionitis: Insights into Inflammatory Responses and Epithelial Barrier Protection
S Yun, SH Kang, J Ryu, K Kim, KY Lee, JJ Lee,International Journal of Molecular Sciences,2025
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Staphylococcal Enterotoxin Gene B Relevant to Atopic Dermatitis Alters the Expression of Kallikreins and β-defensin 2 in HaCaT Cells
X Tian, Q Huang, Z Chen, M Xia, J Liang, Y Liu,scientific reports,2024
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Increasing expression of STING by ERα antagonizes LCN2 downregulation during chronic endometritis
M Chu,Journal of reproductive immunology,2023
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Dynamic changes of Th1/Th2/Th17 cytokines and hBD-2/3 in erosive oral lichen planus patients saliva before and after prednisone acetate treatment
M Xie,Heliyon,2024
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Protective role of human beta-defensin-2 and cathelicidin in high risk close household contacts of pulmonary tuberculosis
S Panda,Clinical Immunology Communications,2023
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Protective role of human beta-defensin-2 and cathelicidin in high risk close household contacts of pulmonary tuberculosis
S Panda,Clinical Immunology Communications,2023
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Evaluation of human β?defensins in the cerebrospinal fluid of suspected meningitis
LK Jaleel,Biomedical Reports,2022
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Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum-like environment
Borrel V, et al,Microbiologyopen,2019
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Salivary human beta defensins affected by oral Candida status in Chinese HIV/AIDS patients undergoing ART
Liu Z.et al,Oral Dis,2018
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MABp1 Targeting Interleukin-1Alpha for Moderate to Severe Hidradenitis Suppurativa not Eligible for Adalimumab: A Randomized Study
Kanni T.et al,J Invest Dermatol,2017
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High Copy Numbers of β-Defensin Cluster on 8p23.1, Confer Genetic Susceptibility, and Modulate the Physical Course of Hidradenitis Suppurativa/Acne Inversa.
Giamarellos-Bourboulis EJ.et al,J Invest Dermatol.,2016
靶點詳情
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功能：
Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria. May act as a ligand for C-C chemokine receptor CCR6. Can bind to both human and mouse CCR6 and induce chemotactic activity of CCR6-expressing cells.
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基因功能參考文獻：
- The encoded peptide has antimicrobial activity against E.coli, S. aureus, and P. aeruginosa. PMID: 9727055
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亞細胞定位：
Secreted.
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蛋白家族：
Beta-defensin family, LAP/TAP subfamily
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組織特異性：
Expressed in the skin and respiratory tract.
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數據庫鏈接：
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