Human α-Glucosidase ELISA Kit

說明書下載

中文名稱：

人α葡萄糖苷酶(α-glucosidase)酶聯免疫試劑盒
貨號：

CSB-E09459h
規格：

96T/48T
價格：

￥3600/￥2500
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人α葡萄糖苷酶(α-glucosidase)酶聯免疫試劑盒（CSB-E09459h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、尿液樣本中的MGAM含量。MGAM即麥芽糖酶-葡糖淀粉酶，在碳水化合物消化中扮演重要角色。研究機制方面，主要聚焦于其在腸道內對淀粉的水解作用，抑制其活性可減少碳水吸收。相關研究有助于開發治療糖尿病、肥胖癥的藥物，以調節血糖和體重。試劑盒檢測范圍為3.12 μU/mL-200 μU/mL，本試劑盒適用于體外科研實驗，可幫助研究者探索α - 葡萄糖苷酶在代謝性疾病模型中的表達變化，評估藥物或天然產物對酶活性的調控作用，或作為生物標志物用于糖代謝相關病理機制的基礎研究。實驗操作兼容多種生物樣本類型，為體外培養細胞、動物模型或臨床前研究提供可靠的定量分析工具，支持從分子機制到功能驗證的多層次研究需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

4-alpha-glucosidase ELISA Kit; Alpha-glucosidase ELISA Kit; Glucan 1 ELISA Kit; Glucoamylase ELISA Kit; Maltase-glucoamylase; intestinal ELISA Kit; MGA ELISA Kit; MGA_HUMAN ELISA Kit; MGAM ELISA Kit; MGAML ELISA Kit
縮寫：

MGAM
Uniprot No.：

O43451
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, urine
檢測范圍：

3.12 μU/mL-200 μU/mL
靈敏度：

0.78 μU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human α-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	95
	Range %	85-103
1:40	Average %	90
	Range %	85-99
1:80	Average %	95
	Range %	91-102
1:100	Average %	89
	Range %	81-97

回收率：

The recovery of human α-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-101
EDTA plasma (n=4)	93	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µU/ml	OD1	OD2	Average	Corrected
200	2.127	2.112	2.120	1.913
100	1.746	1.757	1.752	1.545
50	1.442	1.417	1.430	1.223
25	1.069	1.028	1.049	0.842
12.5	0.835	0.843	0.839	0.632
6.25	0.502	0.521	0.512	0.305
3.12	0.347	0.354	0.351	0.144
0	0.205	0.209	0.207

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.
基因功能參考文獻：
1. Mechanistic Pathway on Human alpha-Glucosidase Maltase-Glucoamylase Unveiled by QM/MM Calculations PMID: 29548257
2. MGAM, or nearby regulatory elements, may be involved in the etiology of oral clefts. PMID: 25776870
3. Starch internal structure modulates its susceptibility to MGAM. The internal branch amounts negatively affect the glucose release rate. PMID: 25037326
4. Findings suggest that C-terminal subunits of recombinant maltase-glucoamylase (MGAM) assists alpha-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies. PMID: 22563462
5. These results suggest that the N-terminal and C-terminal catalytic domains of maltase-glucoamylase differ in their substrate specificities and inhibitor tolerance despite their structural relationship PMID: 22036121
6. we report crystal structures of C-terminal maltase-glucoamylase alone at a resolution of 3.1 angstroms, and in complex with its inhibitor acarbose PMID: 22058037
7. analysis of substrate selectivity of human maltase-glucoamylase and sucrase-isomaltase N-terminal domains PMID: 20356844
8. genetic analysis of MGAM, exon boundaries, and chromosome mapping PMID: 12547908
9. Raw starch granule degradation with recombinanat human MGAM indicates that pancreatic alpha-amylase hydrolysis is not a requirement for native starch digestion in the human small intestine. PMID: 17485087
10. Intestinal maltase-glycoamylase: crystal structure of the N-terminal catalytic subunit and basis of inhibition and substrate specificity. PMID: 18036614
11. Acarbose has been found to improve insulin levels and thus glucose/insulin ratios more effectively in overweight patients compared with nonoverweight patients with PCOS. PMID: 18377903
12. This study reported the first diagnosed Finnish patient with a phenotype compatible with the late-onset form of Pompe disease. Molecular genetic analysis of the GAA gene revealed a novel missense mutation (Y575X),combined with (P545L) mutation. PMID: 19472353
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亞細胞定位：

Apical cell membrane; Single-pass type II membrane protein. Note=Brush border.
蛋白家族：

Glycosyl hydrolase 31 family
組織特異性：

Expressed in small intestine, granulocyte, and kidney but not in salivary gland or pancreas.
數據庫鏈接：

HGNC: 7043

OMIM: 154360

KEGG: hsa:8972

STRING: 9606.ENSP00000447378

UniGene: Hs.122785

Human α-Glucosidase ELISA Kit

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