Horse Prolactin(PRL) ELISA kit

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中文名稱：

馬催乳素(PRL)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL018724HO
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

馬催乳素(PRL)酶聯(lián)免疫試劑盒（CSB-EL018724HO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的PRL含量。PRL即泌乳素，是腺垂體分泌的多肽激素。其分泌受下丘腦調(diào)節(jié)。研究機(jī)制上，它在乳腺發(fā)育、泌乳啟動(dòng)維持等生理過程中發(fā)揮重要作用，異常分泌會(huì)致月經(jīng)不調(diào)、不孕等，在腫瘤發(fā)生發(fā)展等方面也有一定關(guān)聯(lián)，是醫(yī)學(xué)研究重要靶點(diǎn)。試劑盒檢測(cè)范圍為2.5 ng/mL-1000 ng/mL，產(chǎn)品經(jīng)過嚴(yán)格的性能驗(yàn)證，可穩(wěn)定應(yīng)用于馬科動(dòng)物繁殖生理、泌乳調(diào)控機(jī)制、應(yīng)激狀態(tài)評(píng)估等研究領(lǐng)域，為畜牧科研、動(dòng)物內(nèi)分泌研究及生殖健康管理提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
縮寫：

PRL
Uniprot No.：

P12420
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma
檢測(cè)范圍：

2.5 ng/mL-1000 ng/mL
靈敏度：

2.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-101
1:2	Average %	95
	Range %	85-100
1:4	Average %	91
	Range %	89-99
1:8	Average %	93
	Range %	90-100

回收率：

The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-105
EDTA plasma (n=4)	97	88-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
0	2.133	2.121	2.127
2.5	1.516	1.501	1.509
8.75	0.996	1.002	0.999
31.25	0.538	0.555	0.547
187.5	0.341	0.339	0.340
1000	0.162	0.159	0.161

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Theriogenology Wild TL Roth,researchgate,2023
Post-partum concentrations of serum progesterone, oestradiol and prolactin in Arabian mares demonstrating normal maternal behaviour and Arabian mares D. Berlin.et al,The Veterinary Journal,2017

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功能：

Prolactin acts primarily on the mammary gland by promoting lactation.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ecb:100034034

STRING: 9796.ENSECAP00000000120

UniGene: Eca.12783

Horse Prolactin(PRL) ELISA kit

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