Horse Gelsolin(GSN) ELISA kit
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中文名稱(chēng)：馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL009965HO
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒（CSB-EL009965HO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GSN含量。GSN 是一個(gè)靶點(diǎn)。它在細(xì)胞中具有重要作用，與細(xì)胞的運(yùn)動(dòng)、形態(tài)維持等相關(guān)。研究機(jī)制方面，其可能參與調(diào)節(jié)細(xì)胞骨架的動(dòng)態(tài)變化。通過(guò)對(duì)它的深入研究，有望揭示疾病發(fā)生發(fā)展機(jī)制，為開(kāi)發(fā)新的治療方法和藥物提供方向。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL，該產(chǎn)品可為馬屬動(dòng)物細(xì)胞遷移機(jī)制、炎癥相關(guān)疾病模型或肌肉組織再生等基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)支持，適用于實(shí)驗(yàn)室條件下對(duì)生物樣本中GSN蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)及比較分析，為探索其分子功能及調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Equus caballus (Horse)
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樣本類(lèi)型：serum, plasma, tissue homogenates
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檢測(cè)范圍：12.5 ng/mL-800 ng/mL
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靈敏度：6.25 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Metabolism
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線(xiàn)性度：
To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 101 Range % 94-105 1:2000 Average % 89 Range % 82-94 1:4000 Average % 102 Range % 98-106 1:8000 Average % 95 Range % 91-99 -
回收率：
The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-97 EDTA plasma (n=4) 91 87-96 -
標(biāo)準(zhǔn)曲線(xiàn)：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average 800 0.234 0.228 0.231 400 0.462 0.453 0.458 200 0.710 0.684 0.697 100 1.028 1.001 1.015 50 1.276 1.245 1.261 25 1.584 1.532 1.558 12.5 1.683 1.623 1.653 0 1.827 1.789 1.808 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Synovial fluid alpha‐2‐macroglobulin, gelsolin and lubricin distinguish between osteoarthritic and healthy equine joints EJ Secor, SJ Womack, MP Ysebaert,Equine Veterinary Journal,2025
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
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基因功能參考文獻(xiàn)：
- Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
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亞細(xì)胞定位：Cytoplasm, cytoskeleton.
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蛋白家族：Villin/gelsolin family
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數(shù)據(jù)庫(kù)鏈接：
KEGG: ecb:100034186
UniGene: Eca.2517
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