Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

Hamster Putative phospholipase B-like 2(PLBD2) ELISA kit

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中文名稱：

倉鼠假定磷脂酶B樣蛋白2(PLBD2)酶聯(lián)免疫試劑盒
貨號：

CSB-EL018125HA
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

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產(chǎn)品描述：

倉鼠假定磷脂酶B樣蛋白2(PLBD2)酶聯(lián)免疫試劑盒（CSB-EL018125HA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PLBD2含量。倉鼠PLBD2是一種假定的磷脂酶，與IGF2R相互作用，可能參與生長和信號傳導(dǎo)。其磷脂酶活性尚不明確，但研究可能揭示其在細胞穩(wěn)態(tài)和調(diào)節(jié)機制中的作用。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，科研人員可將其應(yīng)用于基礎(chǔ)研究領(lǐng)域，例如探索PLBD2在脂質(zhì)代謝途徑中的功能機制、評估特定疾病模型中該蛋白的表達動態(tài)變化，或用于細胞信號轉(zhuǎn)導(dǎo)相關(guān)實驗的分子標記物檢測。適用于分子生物學(xué)實驗室常規(guī)蛋白定量研究需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

P76, LAMA-like protein 2|PLB homolog 2|lamina ancestor homolog 2|mannose-6-phosphate protein associated protein p76|phospholipase B-like 2 32 kDa form|phospholipase B-like 2 45 kDa form
縮寫：

PLBD2
種屬：

Mesocricetus auratus (Golden hamster)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of hamster PLBD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-105
1:2	Average %	92
	Range %	86-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of hamster PLBD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	96	89-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.349	2.367	2.358	2.227
400	1.975	1.924	1.950	1.819
200	1.408	1.466	1.437	1.306
100	0.912	0.857	0.885	0.754
50	0.555	0.521	0.538	0.407
25	0.363	0.374	0.369	0.238
12.5	0.248	0.259	0.254	0.123
0	0.133	0.129	0.131

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Method of Characterization of Visible and/or Sub-Visible Particles in Biologics X Xu,freepatentsonline,2020
Compositions and methods for detecting and quantifying host cell protein in cell lines and recombinant polypeptide products Feny Gunawan, et al,/,2018

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.