Guinea pig Interleukin 8,IL-8 ELISA Kit

產品詳情

文獻引用 (8) 評價 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠白介素8(IL-8)酶聯免疫試劑盒（CSB-E13096Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8（C-X-C motif chemokine ligand 8）是一種趨化因子，由巨噬細胞和其他細胞產生，具有促進血管生成和調節炎癥反應的功能。其研究機制涉及對中性粒細胞的召集和脫顆粒，以及與多種疾病如癌癥、炎癥和肥胖的關聯。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，廣泛應用于豚鼠模型的感染性疾病機制研究、免疫調節實驗及藥物開發中的炎癥因子動態監測，特別適用于呼吸道感染、皮膚炎癥或腸道疾病等動物模型的病理分析，為科研人員提供穩定可靠的IL - 8濃度檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit
縮寫：

CXCL8
Uniprot No.：

P49113
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.8 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-102
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	88-102
1:8	Average %	97
	Range %	90-104

回收率：

The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-102
EDTA plasma (n=4)	94	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個評價

樣品類型：組織勻漿液肺組織勻漿上清

樣品信息：其他 SPF級豚鼠

稀釋比：沒有稀釋

產品評價：我通過使用CSB-E13096Gp檢測了PR8病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結果十分精準

By 鄧老師

功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
亞細胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
數據庫鏈接：

STRING: 10141.ENSCPOP00000009984