Goat vitamin B12 (VB12) ELISA kit

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產品詳情

產品描述：

山羊維生素B12(VB12)酶聯免疫試劑盒（CSB-EQ028114GO）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VB12含量。VB12是人體必需的維生素之一，在神經系統功能維持、DNA合成等方面發揮關鍵作用。研究中，其機制集中在參與細胞代謝中的甲基轉移反應和催化同型半胱氨酸轉化為甲硫氨酸等過程，對貧血、神經系統疾病等的防治研究有重要意義。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL，適用于科研領域中對山羊VB12代謝機制的探索，如飼料營養評估、動物生理機能研究或疾病模型中VB12動態變化的分析；組織勻漿檢測功能為動物組織水平研究提供便利；為畜牧業、獸醫學及基礎科研領域的VB12相關研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

VB12
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 pg/mL-100 pg/mL
靈敏度：

0.39 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat VB12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	85
	Range %	80-92
1:20	Average %	97
	Range %	90-105
1:40	Average %	97
	Range %	92-104
1:80	Average %	92
	Range %	86-98

回收率：

The recovery of goat VB12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.