Goat interleukin 12,IL-12/P40 ELISA KIT

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

山羊白介素12(IL-12/P40)酶聯免疫試劑盒（CSB-E12103G）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的IL12B含量。IL12B是重要的靶點。IL - 12B編碼p40亞基，與p35共同組成IL - 12，還能和p19組成IL - 23。其研究機制多圍繞IL - 12和IL - 23介導的免疫反應，在自身免疫病、腫瘤等疾病治療研究中受關注，通過調節免疫來探索潛在療法。試劑盒檢測范圍為9.38 pg/mL-600 pg/mL，適用于科研領域探究宿主抗感染免疫機制、疫苗效力評估或炎癥性疾病模型中細胞因子網絡的動態變化，例如可用于研究山羊布魯氏菌病、口蹄疫等傳染性疾病過程中的免疫激活狀態，或在體外實驗中評估免疫刺激劑對IL - 12/P40分泌水平的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL12B ELISA Kit; Interleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
縮寫：

IL12B
Uniprot No.：

P68221
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

9.37 pg/mL-600 pg/mL
靈敏度：

2.34 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	86
	Range %	82-97
1:4	Average %	93
	Range %	85-102
1:8	Average %	95
	Range %	89-105

回收率：

The recovery of goat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
亞細胞定位：

Secreted.
蛋白家族：

Type I cytokine receptor family, Type 3 subfamily
數據庫鏈接：

KEGG: chx:100860845

UniGene: Chi.38710