Goat Immunoglobulin M(IgM) ELISA Kit

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產品詳情

產品描述：

山羊免疫球蛋白M(IgM)酶聯免疫試劑盒（CSB-E13134G）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgM含量。IgM是一種免疫球蛋白，是機體初次體液免疫應答中最早產生的抗體。在人體免疫系統中發揮著重要作用，能識別并結合病原體。研究機制上，主要圍繞其與抗原的結合特性、激活補體系統等方面展開，以探索其在疾病診斷與治療中的應用。試劑盒檢測范圍為24.4 ng/mL-6250 ng/mL，評估疫苗接種后的抗體應答水平、追蹤感染性疾病進程中的免疫動態，或探究營養干預對免疫功能的調控機制，為畜牧科研、獸醫學研究及動物模型構建提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgM
種屬：

Capra hircus (Goat)
樣本類型：

serum, plasma
檢測范圍：

24.4 ng/mL-6250 ng/mL
靈敏度：

24.4 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of goat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	94
	Range %	89-103
1:10000	Average %	89
	Range %	81-99
1:20000	Average %	97
	Range %	89-105
1:40000	Average %	95
	Range %	83-98

回收率：

The recovery of goat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-105
EDTA plasma (n=4)	95	88-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.