Fish thyroid stimulating hormone(TSH) ELISA Kit

Fish thyroid stimulating hormone(TSH) ELISA Kit

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中文名稱：

魚促甲狀腺素(TSH)酶聯(lián)免疫試劑盒
貨號：

CSB-EQ027261FI
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

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產品描述：

魚促甲狀腺素(TSH)酶聯(lián)免疫試劑盒（CSB-EQ027261FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TSH含量。TSH即促甲狀腺激素，是腺垂體分泌的促進甲狀腺生長和機能的激素。其作用機制主要是調控甲狀腺細胞的生長和甲狀腺血液供應，促進甲狀腺上皮細胞增生和甲狀腺血液供應。在臨床研究中，TSH水平可反映甲狀腺功能狀態(tài)，是診斷甲狀腺疾病的重要指標。試劑盒檢測范圍為2.5 μIU/mL-40 μIU/mL，適用于實驗室開展魚類生理研究、內分泌干擾物效應評估、水產養(yǎng)殖健康監(jiān)測等科研場景。該檢測體系兼容多種樣本類型，為研究魚類甲狀腺功能調控機制、環(huán)境污染物對內分泌系統(tǒng)的影響提供可靠工具，特別適用于水生生物毒理學實驗和基礎生物學研究中的甲狀腺相關指標分析。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

tshb ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
縮寫：

TSH
Uniprot No.：

P37240
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

2.5 μIU/mL-40 μIU/mL
靈敏度：

2.5 μIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	96-106
1:2	Average %	89
	Range %	84-94
1:4	Average %	94
	Range %	90-98
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of fish TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-93
EDTA plasma (n=4)	95	89-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
40	0.234	0.228	0.231
20	0.511	0.502	0.507
10	0.888	0.893	0.891
5	1.743	1.727	1.735
2.5	2.557	2.458	2.508

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Perfluorooctanoic acid disrupts thyroid hormone biosynthesis by altering glycosylation of Na+/I? symporter in larval zebrafish Z Cai, G Zhou, X Yu, Y Du, Q Man, WC Wang,Ecotoxicology and Environmental Safety,2025
Thyroid and growth hormone endocrine disruption and mechanisms of homosalate and octisalate using wild-type, thrαa-/-, and dre-miR-499-/- zebrafish embryo/larvae Y Ka, I Lee, K Ji,Ecotoxicology and Environmental Safety,2024
Effects on thyroid hormone disruption by co-exposure to BEMT and TiO2 nanoparticle in embryo-larval zebrafish (Danio rerio) ???,????? ???,2024
Thyroid Hormone and Lipid Metabolism Disruption of Di (2 propylheptyl) Phthalate (DPHP) in Different Life Stages of Zebrafish ???,????? ???,2024
Thyroid endocrine disruption induced by [C8mim] Br: an integrated in vivo, in vitro, and in silico study P Wei,Aquatic toxicology,2023
Long-term low-dose oxytetracycline potentially leads to neurobehavioural changes Y Qiu,Ecotoxicology and environmental safety,2021
Zebrafish Embryonic Exposure to BPAP and Its Relatively Weak Thyroid Hormone-Disrupting Effects S Lee,Toxics,2020
Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae Lei L,Chemosphere,2020
Phenotypic plasticity as an important mechanism of cave colonization and adaptation in Astyanax cavefish Helena Bilandzija,bioRxiv,2019
Waterborne exposure to BPS causes thyroid endocrine disruption in zebrafish larvae Zhang DH.et al,PLoS One,2017
EFFECTS OF ROSEHIP EXTRACTS ON COMMON CARP (CYPRINUS CARPIO L.) TSH AND THYROID GLAND ACTIVITY H眉seyin Polat.et al,Fresenius Environmental Bulletin,2016
Waterborne exposure to bisphenol F causes thyroid endocrine disruption in zebrafish larvae Huang GM. et al,Chemosphere,2016
Mild hypothyroidism improves glucose tolerance in experimental type 2 diabetes Ashwini S et al,Chem Biol Interact,2015

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功能：

Indispensable for the control of thyroid structure and metabolism. May play some role in the biological processes of the immature fishes.
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
組織特異性：

Pituitary gland. Higher levels seen in immature fishes than the mature fishes.
數據庫鏈接：

UniGene: Omy.8084

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.