Fish Interleukin 6,IL-6 ELISA Kit

Fish Interleukin 6,IL-6 ELISA Kit

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中文名稱：

魚白介素6(IL-6)酶聯免疫試劑盒
貨號：

CSB-E13258Fh
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

魚白介素6(IL-6)酶聯免疫試劑盒（CSB-E13258Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-6含量。IL-6是重要的炎癥細胞因子，在免疫調節、造血等生理過程中發揮關鍵作用。異常升高時與多種疾病如自身免疫病、腫瘤等的發生發展密切相關。其研究機制主要聚焦于阻斷IL-6與其受體結合，以抑制下游信號通路從而達到治療目的。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL，本試劑盒適用于水產養殖中魚類健康監測、病原感染模型建立、免疫增強劑效果評估等科研場景，亦可用于比較醫學中脊椎動物免疫系統的進化機制探究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
縮寫：

IL-6
Uniprot No.：

A0S0B0
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-200 pg/mL
靈敏度：

12.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	89
	Range %	82-94
1:4	Average %	103
	Range %	94-107
1:8	Average %	96
	Range %	90-101

回收率：

The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	91-104
EDTA plasma (n=4)	91	84-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.128	0.133	0.131
100	0.269	0.258	0.264
50	0.449	0.431	0.440
25	0.851	0.842	0.847
12.5	1.813	1.783	1.798

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
Interleukin-6 mediates lipopolysaccharide-inhibited irisin secretion in Nile tilapia (Oreochromis niloticus) Y Yan,Fish & shellfish immunology,2023
Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
Responses of Earthworms to Acetamiprid NO Erhunmwunse,researchsquare,2023
Multiple Biomarker Responses in Female Catfish (Clarias Gariepinus) Exposed to Acetaminophen NO Erhunmwunse,Research Square,2021
Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016

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功能：

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
亞細胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
組織特異性：

Expressed in kidney and spleen. Low expression in liver and gills.

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.