Dog Tumor necrosis factor α,TNF-α ELISA Kit
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中文名稱：犬腫瘤壞死因子α (TNF-α )酶聯免疫試劑盒
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貨號：CSB-E11737c
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規格：96T/48T
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價格：￥4200/￥3000
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其他：
產品詳情
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產品描述：犬腫瘤壞死因子α (TNF-α )酶聯免疫試劑盒（CSB-E11737c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。TNF即腫瘤壞死因子，是一種重要的細胞因子。它在炎癥反應、免疫調節等生理病理過程中起關鍵作用。研究機制上，TNF可通過與受體結合，激活多條信號通路，參與細胞存活、凋亡、增殖等調控，其異常與多種疾病如自身免疫病、腫瘤等相關。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產品廣泛應用于動物模型研究，如炎癥性疾病機制探索、免疫抑制劑藥效評估、疫苗免疫原性分析等領域，為犬類基礎醫學研究和生物標志物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：0.156 ng/mL-10 ng/mL
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靈敏度：0.039 ng/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of dog TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 89-105 1:2 Average % 93 Range % 86-99 1:4 Average % 97 Range % 90-105 1:8 Average % 95 Range % 90-100 -
回收率：
The recovery of dog TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 90-105 EDTA plasma (n=4) 95 93-99 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 10 2.211 2.235 2.223 2.078 5 1.672 1.684 1.678 1.533 2.5 1.112 1.135 1.124 0.979 1.25 0.629 0.639 0.634 0.489 0.625 0.373 0.384 0.379 0.234 0.312 0.264 0.277 0.271 0.126 0.156 0.197 0.201 0.199 0.054 0 0.144 0.145 0.145 -
數據處理：
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貨期：3-5 working days
產品評價
相關產品
靶點詳情
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功能：Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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基因功能參考文獻：
- two SNPs of the TNF gene are associated with obesity in Labrador dogs PMID: 26692319
- Fatal outcome from pulmonary hemorrhages in dogs with leptospirosis was associated with low TNF-alpha, high IL-1beta, and high iNOS expression. PMID: 26824356
- This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol. PMID: 25312048
- Tumour necrosis factor (TNF)-alpha expression was observed in vascular endothelial cells in both neoplastic and normal kidney. PMID: 22300704
- The observed HMGB1 release kinetics showed a clearly time-dependent manner with a peak release 24h after TNF-alpha stimulation, while stimulation with IFN-gamma had only small effects on the HMGB1 release. PMID: 22154216
- the TNF-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular cells is mediated through Src- and TACE-dependent EGFR activation. PMID: 21212278
- The replication characteristics of H3N8 influenza virus and its effects on protein expression of canine alveolar macrophages are reported; only TNF-alpha levels were significantly increased 12 h after inoculation. PMID: 19605916
- Inflammatory cytokines and NO may be involved in intraneural inflammatory changes arising from mechanical compression PMID: 16022989
- mRNA and protein structures and identities of the present known mammalian TNFA. PMID: 17573384
- findings suggest that susceptibility to Anal furunculosis in German Shepherd dogs is primarily associated with DLA-DRB1*00101 and any association with the Tumor Necrosis Factor-alpha locus is secondary and is because of linkage disequilibrium . PMID: 19254251
- the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury PMID: 19261619
- The usefulness of HSP27, HSP70, and TNF-alpha as biological markers for canine skin diseases was studied. PMID: 19578946
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亞細胞定位：Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
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蛋白家族：Tumor necrosis factor family
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數據庫鏈接：
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